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Recombinant EN-TEV Protease Protein
- 种属: 烟草蚀纹病毒(Tobacco etch virus(TEV))
- Accession #: P04517
- 表达系统: E. coli
- 蛋白标签: N-His
- 内毒素水平: <1.0 EU/μg
- 生物活性: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
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T4 DNA 聚合酶
- 种属: Escherichia phage T4
- Accession #: P04415
- 表达系统: E. coli
- 生物活性: Catalytic incorporation of 10nmol deoxyribonucleotides (dNTPs) into polynucleotides at 37 ℃ for 30 minutes. The required enzyme quantity is defined as one active unit.
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核酸内切酶 VIII
- 种属: 大肠杆菌(E.coli)
- Accession #: P50465.3
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to cleave 1pmol of a 34 mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10μl in 1 hour at 37℃ in 1X Endonuclease VIII Reaction Buffer containing 10pmol of fluorescently labeled oligonucleotide duplex
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FnCas12a (Cpf1)
- 种属: 土拉弗朗西斯菌亚种.诺维奇达(U112菌株)(Francisella tularensis subsp. novicida (strain U112))
- Accession #: A0Q7Q2.1
- 表达系统: E. coli
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Poly(U)聚合酶
- 种属: 粟酒裂殖酵母(Schizosaccharomyces pombe)
- Accession #: O13833.2
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme that incorporates 1nmol of UMP into RNA in a 50μl volume in 10 minutes at 37ºC.
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蛋白G-微球菌核酸酶 (pG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A/G-微球菌核酸酶 (pAG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A-微球菌核酸酶（pA-MNase）
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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T4 RNA 连接酶 2，截短型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.
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T4 RNA 连接酶 2，截短突变型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
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T4 RNA 连接酶
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: 2C5U_B,P00971
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to catalyze 1nmol of 5'-[32P]-(A)12-18 to a phosphatase-indigestible cyclic product within 30 minutes at 37℃.
