切割、合成、连接和修饰 - RNA相关 - 分子生物学

生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.

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T4 RNA 连接酶 2，截短突变型

规格或纯度 :

≥95%(SDS-PAGE)
200 U/µL

种属: 肠杆菌噬菌体T4(Enterobacteria phage T4) Accession #: P32277.1

表达系统: E. coli

生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.

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Annealing Buffer for RNA Oligos (10X)

规格或纯度 :

for DNA and RNA applications

10X

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AlkB (RNA/DNA 去甲基化酶, Nuclease-free)

规格或纯度 :

20 U/µL

种属: 大肠杆菌K12株(Escherichia coli (strain K12)) Accession #: P05050.1

表达系统: E. coli

生物活性: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.

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