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克隆和蛋白表达
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蛋白G-微球菌核酸酶 (pG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A/G-微球菌核酸酶 (pAG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A-微球菌核酸酶（pA-MNase）
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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T4 RNA 连接酶 2，截短型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.
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T4 RNA 连接酶 2，截短突变型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
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T4 RNA 连接酶
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: 2C5U_B,P00971
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to catalyze 1nmol of 5'-[32P]-(A)12-18 to a phosphatase-indigestible cyclic product within 30 minutes at 37℃.
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T4 gene 32 protein (ssDNA结合蛋白）
- 种属: Escherichia phage T4
- Accession #: P03695
- 表达系统: E. coli
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T3 RNA 聚合酶
- 种属: 肠杆菌噬菌体T3(Enterobacteria phage T3)
- Accession #: YP_009792932.1,P07659
- 表达系统: E. coli
- 生物活性: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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牛痘DNA拓扑异构酶I
- 种属: 痘苗病毒(Vaccinia virus)
- Accession #: P68698.1
- 表达系统: E. coli
- 生物活性: One unit of DNA Topoisomerase I from Vaccinia converts 1 µg of supercoiled closed circular pUC18 DNA to relaxed closed circular form in 30 min at 37°C.
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Bst DNA Polymerase, Large Fragment
- 种属: 嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)
- Accession #: Q45458
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65℃.
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5'端 DNA/RNA 腺苷酰化酶
- 种属: 嗜热菌腺苷水解酶基因(Adenosylase gene of thermophilic bacteria)
- Accession #: 8UCH_A,A0A075LQ94
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.
