When electrophoresis is performed on a sample denatured with buffer, if the sample does not move when the sample volume is high, and if the sample volume is low, the sample runs fast and unevenly, how to solve the problem?

When electrophoresis is performed on a sample denatured with buffer, if the sample does not move when the sample volume is high, and if the sample volume is low, the sample runs fast and unevenly, how to solve the problem?

If the protein bands do not move at a uniform rate, it is recommended to completely dissolve the sample buffer in a warm bath at 37℃ or below before adding the protein samples, mix well with the protein samples and then boil for 10 minutes to completely denature the protein samples. If the protein bands move slowly, check the electrophoresis tank for leakage.