应用
选项
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T4 RNA 连接酶 2,截短型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.
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T4 RNA 连接酶 2,截短突变型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
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5'端 DNA/RNA 腺苷酰化酶
- 种属: 嗜热菌腺苷水解酶基因(Adenosylase gene of thermophilic bacteria)
- Accession #: 8UCH_A,A0A075LQ94
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.
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末端脱氧核糖核酸转移酶 (TdT)
- 种属: 牛(Bovine)
- Accession #: P06526
- 表达系统: E. coli
- 生物活性: One Unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
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末端脱氧核糖核酸转移酶 (TdT)
- 种属: 牛(Bovine)
- Accession #: P06526
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
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