信号通路蛋白
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Recombinant Mouse Leptin ProteinCAS号: 181030-10-4分子式: NA
- 种属: 小鼠(Mouse)
- Accession #: Q544U0
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <1.0 EU/μg
- 生物活性: Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml. Loaded Recombinant Mouse LEPR (C-10His) on HIS1K Biosensor, can bind Recombinant Mouse Leptin with an affinity constant of 0.196 nM as determined in BLI assay.
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Recombinant Human FGF basic/FGF2/bFGF(155aa) ProteinCAS号: 62031-54-3分子式: 分子量: 17kD
- 种属: 人(Human)
- Accession #: P09038
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <0.2 EU/μg
- 生物活性: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
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Recombinant Human TNF-alpha Protein
- 种属: 人(Human)
- Accession #: P01375
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <0.1 EU/μg
- 生物活性: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED₅₀ for this effect is typically <0.05 ng/mL.
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Recombinant Human IGFBP5 Protein
- 种属: 人(Human)
- Accession #: P24593
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <0.1 EU/μg
- 生物活性: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
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Recombinant Mouse Prolactin Protein
- 种属: 小鼠(Mouse)
- Accession #: P06879
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <0.1 EU/μg
- 生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10^6 IU/mg.
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Recombinant Rat Prolactin Protein
- 种属: 大鼠(Rat)
- Accession #: P01237
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <1.0 EU/μg
- 生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
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Recombinant Human HBEGF/DTR Protein
- 种属: 人(Human)
- Accession #: Q99075
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <1.0 EU/μg
- 生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0×106 IU/mg.
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Recombinant Human LC3B Protein
- 种属: 人(Human)
- Accession #: Q9GZQ8
- 表达系统: E. coli
- 蛋白标签: N-His
- 内毒素水平: <1.0 EU/μg
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Recombinant Human KGF/FGF-7 Protein
- 种属: 人(Human)
- Accession #: P21781
- 表达系统: E. coli
- 蛋白标签: No tag
- 内毒素水平: <1.0 EU/μg
- 生物活性: Measured in a cell proliferation assay using MCF-7. The ED50 for this effect is typically <2.0 ng/mL.
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Recombinant Human HMGB1 Protein
- 种属: 人(Human)
- Accession #: P09429
- 表达系统: E. coli
- 蛋白标签: N-His
- 内毒素水平: <1.0 EU/μg
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Recombinant Human TAGLN/Transgelin Protein
- 种属: 人(Human)
- Accession #: Q01995
- 表达系统: E. coli
- 蛋白标签: N-His
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Recombinant EN-TEV Protease Protein
- 种属: 烟草蚀纹病毒(Tobacco etch virus(TEV))
- Accession #: P04517
- 表达系统: E. coli
- 蛋白标签: N-His
- 内毒素水平: <1.0 EU/μg
- 生物活性: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
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