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  1. Recombinant Mouse Leptin Protein
    CAS号: 181030-10-4
    分子式: NA       
    • 种属: 小鼠(Mouse)
    • Accession #: Q544U0
    • 表达系统: E. coli
    • 蛋白标签: No tag
    • 内毒素水平: <1.0 EU/μg
    • 生物活性: Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml. Loaded Recombinant Mouse LEPR (C-10His) on HIS1K Biosensor, can bind Recombinant Mouse Leptin with an affinity constant of 0.196 nM as determined in BLI assay.
  2. Recombinant EN-TEV Protease Protein
    • 种属: 烟草蚀纹病毒(Tobacco etch virus(TEV))
    • Accession #: P04517
    • 表达系统: E. coli
    • 蛋白标签: N-His
    • 内毒素水平: <1.0 EU/μg
    • 生物活性: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
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