Recombinant Mouse Leptin Protein

无动物源

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

功能和特点

表达系统: E. coli
Accession #: Q544U0
蛋白标签: No tag
生物活性: Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml. Loaded Recombinant Mouse LEPR (C-10His) on HIS1K Biosensor, can bind Recombinant Mouse Leptin with an affinity constant of 0.196 nM as determined in BLI assay.
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
L329647-1mg	1mg	现货

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点击查看蛋白信息: LEP

Accession#: Q544U0 (1) Gene ID: 16846 (1) LEP (3) Leptin (4)

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基本描述

产品名称	Recombinant Mouse Leptin Protein
别名	重组小鼠Leptin蛋白 \| 重组小鼠瘦素蛋白
英文别名	FLJ94114 \| LEP \| leptin (murine obesity homolog) \| leptin (obesity homolog, mouse) \| Leptin \| OB \| Obese protein \| obese, mouse, homolog of \| Obesity factor \| OBOBS \| obese
规格或纯度	ActiBioPure™, 生物活性, 无动物源, 无载体, ≥95%(SDS-PAGE)
生化机理	May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Leptin, known as the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite in order to achieve energy homeostasis. In obesity, a decreased sensitivity to leptin occurs, resulting in an inability to detect satiety despite high energy stores.
生物活性	Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml. Loaded Recombinant Mouse LEPR (C-10His) on HIS1K Biosensor, can bind Recombinant Mouse Leptin with an affinity constant of 0.196 nM as determined in BLI assay.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	小鼠(Mouse)
氨基酸	22-167 aa
序列	VPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	Q544U0
来源	重组表达
预测分子量	16.1 KDa
SDS-PAGE	14 KDa, under reducing conditions.

图片

Protein Bioactivity: Recombinant Mouse Leptin Protein (L329647)

combinant Mouse Leptin Protein (L329647) - Protein Bioactivity
Immobilized Recombinant Mouse LEPR Protein at 5 μg/mL can bind Recombinant Mouse Leptin Protein (L329647). The ED50 for this effect is 1.16 ng/mL.

SDS-PAGE: Recombinant Mouse Leptin Protein (L329647)

Recombinant Mouse Leptin Protein (L329647) - SDS-PAGE
2 μg/lane of Recombinant Mouse Leptin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14 kDa.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM PB, 300mM NaCl, pH7.5.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	长期储存-20℃（12个月）；复溶后建议分装，避免反复冻融。
CAS编号和信息	181030-10-4
分子类型	蛋白质

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批号(Lot Number)	证书类型	日期	货号
ZJ24F1113297	分析证书	24-11-04	L329647

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