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克隆和蛋白表达
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AlkB (RNA/DNA 去甲基化酶, Nuclease-free)
- 种属: 大肠杆菌K12株(Escherichia coli (strain K12))
- Accession #: P05050.1
- 表达系统: E. coli
- 生物活性: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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热敏磷酸酶
- 种属: 南极细菌TAB5(Antarctic bacterium TAB5)
- Accession #: 2W5W_B,Q9KWY4
- 表达系统: E. coli
- 生物活性: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.
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Cre重组酶
- 种属: 大肠杆菌噬菌体P1
- Accession #: Q5QBM7
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to achieve the maximum site-specific recombination of 250ng of control DNA in 30 minutes at 37ºC in a 50μl reaction system. The maximum recombination can be analyzed by agarose gel electrophoresis or by screening on the corresponding resistance plates after transformation of the reactions.
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Klenow Fragment, Exo-
- 种属: 大肠杆菌(E.coli)
- Accession #: 2KFZ_A,P00582
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.
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SP6 RNA 聚合酶
- 种属: 肠杆菌噬菌体SP6(Enterobacteria phage SP6)
- Accession #: Q7Y5N4
- 表达系统: E. coli
- 生物活性: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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T7 Endonuclease I
- 种属: Escherichia phage T7
- Accession #: P00641.1
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.
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末端脱氧核糖核酸转移酶 (TdT)
- 种属: 牛(Bovine)
- Accession #: P06526
- 表达系统: E. coli
- 生物活性: One Unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
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末端脱氧核糖核酸转移酶 (TdT)
- 种属: 牛(Bovine)
- Accession #: P06526
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
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T4 UvsX 重组酶
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: A0A023ZVM8
- 表达系统: E. coli
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大肠杆菌聚合酶 I 大片段
- 种属: 大肠杆菌(E.coli)
- Accession #: 2KFZ_A,P00582
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.
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重组核糖核酸酶HII
- 种属: 大肠杆菌K12株(Escherichia coli (strain K12))
- Accession #: P10442
- 表达系统: E. coli
- 生物活性: One unit refers to the amount of enzyme required to cleave 1 nmol per minute of a synthetic DNA/RNA hybrid double stranded substrate containing a single rC at 37 ° C
