SalI 是一种 Type IIP 类的限制性内切酶，可识别 G^TCGAC 回文序列，适用于酶切质粒、PCR 产物等，可在 15-30 min 内完成 DNA 切割反应。SalI 和 10×Cut Reaction Buffer 均添加了重组白蛋白（rAlbumin），确保了产品的安全性及稳定性。

L397686	Component	200T	Storage
L397686A	SalI	200 μL	-20°C. Avoid freeze/thaw cycle
L397686B	10×Cut Reaction Buffer	1.6 mL	-20°C. Avoid freeze/thaw cycle

来源

E.coli

酶活定义

在 50 μl 反应体系中，37℃下，1小时内酶切1 μg λDNA （HindIII 酶切）所需的酶量定义为 1 个活力单位（U）。

酶切位点

5’…G↓TCGAC…3’
3’…CAGCT↑G…5’

产品使用步骤

（1）配置反应体系，如下表所示

Component	Volume
DNA	To 1 μg
10×Cut Reaction Buffer	5 μl
SalI	1 μl*
Nuclease-free Water	To 50 μl

（2）混匀，于37℃孵育15-30min。

（3）65°C孵育 20 min，终止反应。

注:*Sal 最后加入体系中，一般50u 反应体系加入1 μl的Sall，也可根据实验进行调整，建议酶的加入量不超过总体系的 10%，避免因甘油含量过高产生星活性。

注意事项
(1)Sall 对 Dam、Dcm 甲基化不敏感；对 CpG 甲基化敏感。
(2)在酶切反应前，尽量确保 DNA 不含苯酚、氯仿、酒精、EDTA等杂质，以免影响酶切效果。
(3)本产品仅作科学研究使用，不得用于其它用途。

SalI is a Type IIP restriction enzyme, which can recognize G^TCGAC palindromic sequence, and is suitable for enzyme cleavage plasmid, PCR products, etc., and can complete DNA cleavage reaction within 15-30 min. Recombinant albumin was added to both SalI and 10×Cut Reaction Buffer to ensure the safety and stability of the product.

Source
E.coli

Enzyme Activity Definition

In a 50 μl reaction system, the amount of enzyme required for enzymatic digestion of 1 μg λDNA (HindIII enzyme digestion) within 1 hour at 37℃ is defined as 1 unit of activity (U).

Restriction Enzyme CuttingSite
5’…G↓TCGAC…3’
3’…CAGCT↑G…5’

Protocol
(1) Configure the reaction system, as shown in the following table

Component	volume
DNA	To 1μg
10×Cut Reaction Buffer	5 μl
SalI	1 μl*
Nuclease-free Water	To 50μl

(2) Mix well and incubate at 37℃ for 15-30min.
(3) Incubation at 65°C for 20 min terminated the reaction.
Note :*Sal is finally added to the system, the general 50u reaction system adds 1μl of Sall, and can also be adjusted according to the experiment, it is recommended that the amount of enzyme added does not exceed 10% of the total system to avoid star activity due to high glycerol content.

Matters needing attention
(1)Sall is not sensitive to Dam and Dcm methylation; It is sensitive to CpG methylation.
(2) Before the enzyme digestion reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, so as not to affect the effect of enzyme digestion.
(3) This product is for scientific research only and shall not be used for other purposes.

安全和危险性（GHS）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

SalI

库存信息

基本描述

名称和识别符