L359050	Component	1 KU	5 KU	Storage
L359050A	BsaI	50 μL	250 μL	-20°C. Avoid freeze/thaw cycle
L359050B	10×Cut Reaction Buffer	300 μL	1.5 mL	-20°C. Avoid freeze/thaw cycle

来源

E.coli

酶切位点

5’…GGTCTC(N)1↓…3’
3’…CCAGAG(N)5↑…5’

酶活定义

37℃条件下，在 50 μl 反应体系中，1 h 内完全消化 1 μg λDNA Dcm-HindIII fragments 所需的酶量定义为 1 个活力单位（U）。

产品使用步骤

实例一：DNA 快速酶切

（1）按照下表所示配置混合溶液：

Component	基因组 DNA	PCR 产物	质粒 DNA
RNase-free Water	To 50 μl	To 30 μl	To 20 μl
10× Cut Reaction Buffer	5 μl	3 μl	2 μl
DNA	10 μl (5 μg)	10 μl (0.2 μg)	2 μl (1 μg)
BsaI	5 μl	1 μl	1 μl

（2）轻柔吸打混匀，切勿振荡涡旋，然后瞬时离心。

（3）37℃反应 15 min（质粒），或15-30min（PCR产物），或 30-60 min（基因组DNA）。

（4）80℃反应 20 min 可使酶失活，终止反应（可选）。

实例二：适用于质粒的扩大反应体系

RNase-free Water	To 20 μl	To 20 μl	To 30 μl	To 40 μl	To 50 μl
10× Cut Reaction Buffer	2μl	2 μl	3 μl	4 μl	5 μl
质粒	1 μg	2 μg	3 μg	4 μg	5 μg
BsaI	1 μl	2 μl	3 μl	4 μl	5 μl

注意事项

（1）Dcm、CpG 和 EcoB I 的甲基化修饰会损害本品的切割活性。

（2）未纯化的 PCR 产物具备一定的离子强度，此外，由于 DNA 聚合酶同时具有外切酶活性，会影响酶切产物，因此如下一步需进行克隆等操作，建议酶切前对 PCR 产物进行纯化。

（3）本产品仅作科学研究使用，不得用于其它用途

Restriction enzymes are a class of endonuclease enzymes that recognize specific sequences of deoxyribonucleotides and cut the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI is an IIS restriction enzyme that recognizes non-palindromic sequences and cuts sticky ends outside the recognition sequence.

Source
E.coli

Restriction Enzyme CuttingSite
5’…GGTCTC(N)1↓…3’
3’…CCAGAG(N)5↑…5’

Enzyme Activity Definition
The amount of enzyme required to fully digest 1 μg λDNA Dcm-HindIII fragments within 1 h in a 50 μl reaction system at 37℃ is defined as 1 activity unit (U).

Protocol
Example 1: DNA rapid enzyme digestion
(1) Configure the mixture as shown in the following table:

Component	Genome DNA	PCR Product	Plasmid DNA
RNase-free Water	To 50 μl	To 30 μl	To 20 μl
10× Cut Reaction Buffer	5 μl	3 μl	2 μl
DNA	10 μl (5 μg)	10 μl (0.2 μg)	2 μl (1 μg)
BsaI	5 μl	1 μl	1 μl

(2) Gently mix, do not oscillate the vortex, and then instantaneous centrifugation.
(3) Reaction at 37℃ for 15 min (plasmid), or 15-30min (PCR product), or 30-60 min (genomic DNA).
(4) Reaction at 80℃ for 20 min can deactivate the enzyme and terminate the reaction (optional).

Example 2: It is suitable for extended reaction system of plasmids

RNase-free Water	To 20 μl	To 20 μl	To 30 μl	To 40 μl	To 50 μl
10× Cut Reaction Buffer	2μl	2 μl	3 μl	4 μl	5 μl
Plasmid	1 μg	2 μg	3 μg	4 μg	5 μg
BsaI	1 μl	2 μl	3 μl	4 μl	5 μl

Cautions
(1) The methylation of Dcm, CpG and EcoB I will impair the cutting activity of this product.
(2) Unpurified PCR products have a certain ionic strength. In addition, since DNA polymerase also has exonuclidene activity, it will affect the enzyme digestion products. Therefore, cloning and other operations need to be performed in the following step.
(3) This product is for scientific research only and shall not be used for other purposes

安全和危险性（GHS）

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

BsaI

库存信息

库存信息

基本描述

名称和识别符