基本信息

产品名称	CD44 Mouse mAb
别名	吞噬细胞糖蛋白-1 \| 同源细胞黏附分子 \| 细胞表面I型跨膜糖蛋白 \| 同源细胞黏附分子抗体 \| 同源细胞黏附分子小鼠单克隆抗体 \| 小鼠抗人同源细胞黏附分子抗体 \| 吞噬细胞糖蛋白-1抗体 \| 吞噬细胞糖蛋白-1小鼠单克隆抗体 \| 小鼠抗人吞噬细胞糖蛋白-1抗体 \| 细胞表面I型跨膜糖蛋白抗体 \| 细胞表面I型跨膜糖蛋白小鼠单克隆抗体 \| 小鼠抗人细胞表面I型跨膜糖蛋白抗体 \| CD44抗体 \| CDW44抗体 \| LHR抗体 \| MDU2抗体 \| MDU3抗体 \| MIC4抗体
英文别名	LHR antibody \| BA-1 antibody \| CD 44 antibody \| CD44 antibody \| CD44 antigen antibody \| CD44 molecule (Indian blood group) antibody \| CD44 molecule antibody \| CD44_HUMAN antibody \| CDw44 antibody \| CDW44 antigen antibody \| Cell surface glycoprotein CD44 a
规格或纯度	ExactAb™, 无载体, 已验证, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.
宿主种属	小鼠(Mouse)
特异性	Human CD44
种属反应性	人(Human)
免疫原	Recombinant protein corresponding to Human CD44
阳性对照	WB: HeLa whole cell lysates Flow Cytometry: HeLa and A549 cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

图片

Western Blot: CD44 Mouse mAb (Ab214006)

CD44 Mouse mAb (Ab214006) - Western Blot
All lanes: CD44 Mouse Antibody (Ab214006) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 82 kDa
Observed band size: 79 kDa
Exposure time: 60.0 s

Flow Cytometry: CD44 Mouse mAb (Ab214006)

CD44 Mouse mAb (Ab214006) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with CD44 Mouse mAb (Ab214006) (Red) at 0.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue).

Flow Cytometry: CD44 Mouse mAb (Ab214006)

CD44 Mouse mAb (Ab214006) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with CD44 Mouse mAb (Ab214006) (Red) at 0.25 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue).

Flow Cytometry: CD44 Mouse mAb (Ab214006)

CD44 Mouse mAb (Ab214006) - Flow Cytometry 
Flow Cytometry analysis of A549 cells stained with CD44 Mouse mAb (Ab214006) (Red) at 0.125 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue).