基本信息

产品名称	Recombinant CD8 Antibody (AF700)
别名	CD8单克隆抗体 \| Anti-CD8a抗体 \| CD8a抗体 \| CD8a单抗
英文别名	CD8a \| CD8 antigen, alpha polypeptide (p32) \| CD8 \| CD8a molecule \| Leu2 T-lymphocyte antigen \| LEU2 \| MAL \| OKT8 T-cell antigen \| p32 \| T cell co-receptor \| T8 T-cell antigen \| T-cell antigen Leu2 \| T-cell surface glycoprotein CD8 alpha chain \| T-lymphoc
规格或纯度	ExactAb™, 已验证, 5 μL/test
生化机理	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
宿主种属	小鼠(Mouse)
特异性	CD8
种属反应性	人(Human)
免疫原	Tissue, cells or virus.
阳性对照	Flow: Human peripheral blood lymphocytes
偶联	AF700
Excitation(nm)	Red Laser(633nm)
Emission(nm)	719nm
Ex/Em(nm)	Ex:696nm;Em:719nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: Recombinant CD8 Antibody (AF700) (Ab213302)

Recombinant CD8 Antibody (AF700) (Ab213302) - Flow Cytometry
Flow cytometry analysis of CD8 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8 Antibody (AF700) (Ab213302) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF700) (Ab190248).

Flow Cytometry: Recombinant CD8 Antibody (AF700) (Ab213302)

Recombinant CD8 Antibody (AF700) (Ab213302) - Flow Cytometry
Flow cytometry analysis of CD8 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8 Antibody (AF700) (Ab213302) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF700) (Ab190248). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

CD8A Tbio T 细胞表面糖蛋白 CD8 α 链(T-cell surface glycoprotein CD8 alpha chain) (0 活性数据)

点击查看靶蛋白信息： CD8A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

应用

应用名称	稀释比例
Flow Cytometry	5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.