基本信息

产品名称	Recombinant CD45 Antibody (AF405)
别名	白细胞共同抗原CD45抗体 \| CD45抗体
英文别名	B220 \| CD45 \| CD45 antigen \| CD45R \| EC 3.1.3.48 \| GP180 \| LCA \| L-CA \| Leukocyte common antigen \| LY5 \| protein tyrosine phosphatase \| PTPRC \| receptor-type tyrosine-protein phosphatase C \| T200 Glycoprotein \| T200 leukocyte common antigen \| T200 \| B220
规格或纯度	ExactAb™, 已验证, Ex:401nm, Em:422nm, 5 μL/test
生化机理	Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
宿主种属	小鼠(Mouse)
特异性	CD45
种属反应性	人(Human)
免疫原	Human PBMC
阳性对照	Flow: Human peripheral blood lymphocytes
偶联	AF405
Excitation(nm)	Violet Laser(405nm)
Emission(nm)	422nm
Ex/Em(nm)	Ex:401nm;Em:422nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: Recombinant CD45 Antibody (AF405) (Ab213128)

Recombinant CD45 Antibody (AF405) (Ab213128) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant Recombinant CD45 Antibody (AF405) (Ab213128) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).

Flow Cytometry: Recombinant CD45 Antibody (AF405) (Ab213128)

Recombinant CD45 Antibody (AF405) (Ab213128) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (AF405) (Ab213128) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

PTPRC Tchem 受体型酪氨酸蛋白磷酸酶C(Receptor-type tyrosine-protein phosphatase C) (0 活性数据)

点击查看靶蛋白信息： PTPRC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.