基本信息

产品名称	Recombinant CD45 Antibody (PE-Cy5)
别名	蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体 \| Anti-CD45抗体 \| CD45抗体 \| CD45单抗
英文别名	B220 \| CD45 \| CD45 antigen \| CD45R \| EC 3.1.3.48 \| GP180 \| LCA \| L-CA \| Leukocyte common antigen \| LY5 \| protein tyrosine phosphatase \| PTPRC \| receptor-type tyrosine-protein phosphatase C \| T200 Glycoprotein \| T200 leukocyte common antigen \| T200 \| B220
规格或纯度	ExactAb™, 已验证, Ex:565nm, Em:670nm, 5 μL/test
生化机理	Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
宿主种属	小鼠(Mouse)
特异性	CD45
种属反应性	人(Human)
免疫原	Peripheral blood mononuclear cells
阳性对照	Flow: Human peripheral blood lymphocytes
偶联	PE-Cy5
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	670nm
Ex/Em(nm)	Ex:565nm;Em:670nm

产品属性

克隆类型	重组抗体
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: Recombinant CD45 Antibody (PE-Cy5) (Ab213121)

Recombinant CD45 Antibody (PE-Cy5) (Ab213121) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45 Antibody (PE-Cy5) (Ab213121) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy5) (Ab190078).

Flow Cytometry: Recombinant CD45 Antibody (PE-Cy5) (Ab213121)

Recombinant CD45 Antibody (PE-Cy5) (Ab213121) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (PE-Cy5) (Ab213121) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

PTPRC Tchem 受体型酪氨酸蛋白磷酸酶C(Receptor-type tyrosine-protein phosphatase C) (0 活性数据)

点击查看靶蛋白信息： PTPRC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.