CD45RA Mouse mAb (FITC)

已验证

ExactAb™

无叠氮钠

Ex:498nm

Em:517nm

5μL/test

应用:

Flow Cytometry

功能和特点

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: HI100
偶联: FITC

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab179696-10T	10T	现货
Ab179696-25T	25T	现货
Ab179696-100T	100T	现货

查看相关系列

点击查看靶蛋白信息: PTPRC

Accession#: P08575 (9) CD45 (66) CD45RA (6) FITC (176) Gene ID: 5788 (11) PTPRC (66) 流式细胞术 (1161)

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基本信息

产品名称	CD45RA Mouse mAb (FITC)
别名	异硫氰酸荧光素标记的小鼠抗人CD45RA抗体 \| CD45RA 小鼠单克隆抗体 (FITC) \| CD45RA 小鼠单抗 (FITC)
英文别名	EC 3.1.3.48 \| L-CA \| Leukocyte common antigen \| T200 \| B220 \| CD_antigen: CD45 \| CD45 antigen \| CD45 \| CD45R \| EC 3.1.3.48 \| EC:3.1.3.48 \| GP180 \| LCA \| L-CA \| Leukocyte common antigen \| LY5 \| protein tyrosine phosphatase, receptor type, c polypeptide \| P
规格或纯度	ExactAb™, 已验证, 无叠氮钠, Ex:498nm, Em:517nm, 5μL/test
宿主种属	小鼠(Mouse)
特异性	CD45RA
种属反应性	人(Human)
免疫原	Low buoyancy density human lymphocytes
阳性对照	Flow: Raji
偶联	FITC
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	517nm
Ex/Em(nm)	Ex:498nm;Em:517nm

产品属性

克隆类型	单克隆抗体
克隆号	HI100
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5μL/test
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	未稀释的抗体溶液应保存在 2°C 至 8°C 之间，避免长时间暴露在光线下。切勿冷冻。
分子类型	抗体

图片

Flow Cytometry: CD45RA Mouse mAb (FITC) (Ab179696)

CD45RA Mouse mAb (FITC) (Ab179696) - Flow Cytometry
Flow cytometry analysis of CD45RA expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with CD45RA Mouse mAb (FITC) (Ab179696) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

PTPRC Tchem 受体型酪氨酸蛋白磷酸酶C(Receptor-type tyrosine-protein phosphatase C) (0 活性数据)

点击查看靶蛋白信息： PTPRC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	日期	货号
ZJ24F0303741	分析证书	24-03-27	Ab179696
ZJ24F0303740	分析证书	24-03-27	Ab179696
ZJ24F0303739	分析证书	24-03-27	Ab179696

可替换产品

参考文献

1. Chen YT, Seto CT.. (2002) Divalent and trivalent alpha-ketocarboxylic acids as inhibitors of protein tyrosine phosphatases.. J Med Chem, 45 (18): (3946-3952). [PMID:12190316] [10.1021/jm020093q]
2. Andersen HS, Olsen OH, Iversen LF, Sørensen AL, Mortensen SB, Christensen MS, Branner S, Hansen TK, Lau JF, Jeppesen L, Moran EJ, Su J, Bakir F, Judge L, Shahbaz M, Collins T, Vo T, Newman MJ, Ripka WC, Møller NP.. (2002) Discovery and SAR of a novel selective and orally bioavailable nonpeptide classical competitive inhibitor class of protein-tyrosine phosphatase 1B.. J Med Chem, 45 (20): (4443-4459). [PMID:12238924] [10.1021/jm0209026]
3. He Y, Liu S, Menon A, Stanford S, Oppong E, Gunawan AM, Wu L, Wu DJ, Barrios AM, Bottini N et al.. (2013) A potent and selective small-molecule inhibitor for the lymphoid-specific tyrosine phosphatase (LYP), a target associated with autoimmune diseases.. J Med Chem, 56 (12): (4990-5008). [PMID:23713581]
4. Yu X, Sun JP, He Y, Guo X, Liu S, Zhou B, Hudmon A, Zhang ZY. (2007) Structure, inhibitor, and regulatory mechanism of Lyp, a lymphoid-specific tyrosine phosphatase implicated in autoimmune diseases.. Proc Natl Acad Sci USA, 104 (50): (19767-72). [PMID:18056643]
5. Stanford SM, Krishnamurthy D, Falk MD, Messina R, Debnath B, Li S, Liu T, Kazemi R, Dahl R, He Y et al.. (2011) Discovery of a novel series of inhibitors of lymphoid tyrosine phosphatase with activity in human T cells.. J Med Chem, 54 (6): (1640-54). [PMID:21341673]
6. Katherine A Rawls,P Therese Lang,Jun Takeuchi,Shinichi Imamura,Tyler D Baguley,Christoph Grundner,Tom Alber,Jonathan A Ellman. (2009-11-06) Fragment-based discovery of selective inhibitors of the Mycobacterium tuberculosis protein tyrosine phosphatase PtpA.. Bioorganic & medicinal chemistry letters, 19 ((24)): (6851-6854). [PMID:19889539]
7. Dufresne C, Roy P, Wang Z, Asante-Appiah E, Cromlish W, Boie Y, Forghani F, Desmarais S, Wang Q, Skorey K, Waddleton D, Ramachandran C, Kennedy BP, Xu L, Gordon R, Chan CC, Leblanc Y.. (2004) The development of potent non-peptidic PTP-1B inhibitors.. Bioorg Med Chem Lett, 14 (4): (1039-1042). [PMID:15013019] [10.1016/j.bmcl.2003.11.048]
8. Wilson DP, Wan ZK, Xu WX, Kirincich SJ, Follows BC, Joseph-McCarthy D, Foreman K, Moretto A, Wu J, Zhu M, Binnun E, Zhang YL, Tam M, Erbe DV, Tobin J, Xu X, Leung L, Shilling A, Tam SY, Mansour TS, Lee J.. (2007) Structure-based optimization of protein tyrosine phosphatase 1B inhibitors: from the active site to the second phosphotyrosine binding site.. J Med Chem, 50 (19): (4681-4698). [PMID:17705360] [10.1021/jm0702478]
9. Han Y, Belley M, Bayly CI, Colucci J, Dufresne C, Giroux A, Lau CK, Leblanc Y, McKay D, Therien M, Wilson MC, Skorey K, Chan CC, Scapin G, Kennedy BP.. (2008) Discovery of [(3-bromo-7-cyano-2-naphthyl)(difluoro)methyl]phosphonic acid, a potent and orally active small molecule PTP1B inhibitor.. Bioorg Med Chem Lett, 18 (11): (3200-3205). [PMID:18477508] [10.1016/j.bmcl.2008.04.064]
10. Cosconati S, Hong JA, Novellino E, Carroll KS, Goodsell DS, Olson AJ.. (2008) Structure-based virtual screening and biological evaluation of Mycobacterium tuberculosis adenosine 5'-phosphosulfate reductase inhibitors.. J Med Chem, 51 (21): (6627-6630). [PMID:18855373] [10.1021/jm800571m]
11. Yu WM, Guvench O, Mackerell AD, Qu CK.. (2008) Identification of small molecular weight inhibitors of Src homology 2 domain-containing tyrosine phosphatase 2 (SHP-2) via in silico database screening combined with experimental assay.. J Med Chem, 51 (23): (7396-7404). [PMID:19007293] [10.1021/jm800229d]
12. Vidović D, Schürer SC.. (2009) Knowledge-based characterization of similarity relationships in the human protein-tyrosine phosphatase family for rational inhibitor design.. J Med Chem, 52 (21): (6649-6659). [PMID:19810703] [10.1021/jm9008899]
13. Wu S, Vossius S, Rahmouni S, Miletic AV, Vang T, Vazquez-Rodriguez J, Cerignoli F, Arimura Y, Williams S, Hayes T, Moutschen M, Vasile S, Pellecchia M, Mustelin T, Tautz L.. (2009) Multidentate small-molecule inhibitors of vaccinia H1-related (VHR) phosphatase decrease proliferation of cervix cancer cells.. J Med Chem, 52 (21): (6716-6723). [PMID:19888758] [10.1021/jm901016k]
14. Zhang X, He Y, Liu S, Yu Z, Jiang ZX, Yang Z, Dong Y, Nabinger SC, Wu L, Gunawan AM, Wang L, Chan RJ, Zhang ZY.. (2010) Salicylic acid based small molecule inhibitor for the oncogenic Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2).. J Med Chem, 53 (6): (2482-2493). [PMID:20170098] [10.1021/jm901645u]
15. Vang T, Xie Y, Liu WH, Vidović D, Liu Y, Wu S, Smith DH, Rinderspacher A, Chung C, Gong G, Mustelin T, Landry DW, Rickert RC, Schürer SC, Deng SX, Tautz L.. (2011) Inhibition of lymphoid tyrosine phosphatase by benzofuran salicylic acids.. J Med Chem, 54 (2): (562-571). [PMID:21190368] [10.1021/jm101004d]
16. Yu ZH, Chen L, Wu L, Liu S, Wang L, Zhang ZY.. (2011) Small molecule inhibitors of SHP2 tyrosine phosphatase discovered by virtual screening.. Bioorg Med Chem Lett, 21 (14): (4238-4242). [PMID:21669525] [10.1016/j.bmcl.2011.05.078]
17. He Y, Xu J, Yu ZH, Gunawan AM, Wu L, Wang L, Zhang ZY.. (2013) Discovery and evaluation of novel inhibitors of mycobacterium protein tyrosine phosphatase B from the 6-Hydroxy-benzofuran-5-carboxylic acid scaffold.. J Med Chem, 56 (3): (832-842). [PMID:23305444] [10.1021/jm301781p]
18. Liu W, Yu B, Xu G, Xu WR, Loh ML, Tang LD, Qu CK.. (2013) Identification of cryptotanshinone as an inhibitor of oncogenic protein tyrosine phosphatase SHP2 (PTPN11).. J Med Chem, 56 (18): (7212-7221). [PMID:23957426] [10.1021/jm400474r]
19. Baguley TD, Xu HC, Chatterjee M, Nairn AC, Lombroso PJ, Ellman JA.. (2013) Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.. J Med Chem, 56 (19): (7636-7650). [PMID:24083656] [10.1021/jm401037h]
20. Tautz L, Senis YA, Oury C, Rahmouni S.. (2015) Perspective: Tyrosine phosphatases as novel targets for antiplatelet therapy.. Bioorg Med Chem, 23 (12): (2786-2797). [PMID:25921264] [10.1016/j.bmc.2015.03.075]
21. He R, Bai Y, Yu ZH, Wu L, Gunawan AM, Zhang ZY.. (2014) Diversity-Oriented Synthesis for Novel, Selective and Drug-like Inhibitors for a Phosphatase from Mycobacterium Tuberculosis.. Medchemcomm, 5 (10): (1496-1499). [PMID:25505942] [10.1039/c4md00099d]
22. Haftchenary S, Jouk AO, Aubry I, Lewis AM, Landry M, Ball DP, Shouksmith AE, Collins CV, Tremblay ML, Gunning PT.. (2015) Identification of Bidentate Salicylic Acid Inhibitors of PTP1B.. ACS Med Chem Lett, 6 (9): (982-986). [PMID:26396684] [10.1021/acsmedchemlett.5b00171]
23. Li Y, Xia G, Guo Q, Wu L, Chen S, Yang Z, Wang W, Zhang ZY, Zhou X, Jiang ZX.. (2016) Design, synthesis and evaluation of novel ¹⁹F magnetic resonance sensitive protein tyrosine phosphatase inhibitors.. Medchemcomm, 7 (8): (1672-1680). [PMID:27529021] [10.1039/C6MD00277C]
24. Abdjul DB, Yamazaki H, Kanno SI, Wewengkang DS, Rotinsulu H, Sumilat DA, Ukai K, Kapojos MM, Namikoshi M.. (2017) Furanoterpenes, new types of protein tyrosine phosphatase 1B inhibitors, from two Indonesian marine sponges, Ircinia and Spongia spp.. Bioorg Med Chem Lett, 27 (5): (1159-1161). [PMID:28189419] [10.1016/j.bmcl.2017.01.071]
25. He R,Wang J,Yu ZH,Zhang RY,Liu S,Wu L,Zhang ZY. (2016) Inhibition of Low Molecular Weight Protein Tyrosine Phosphatase by an Induced-Fit Mechanism.. J Med Chem, 59 (19.0): (9094-9106). [PMID:27676368] [10.1021/acs.jmedchem.6b00993]
26. Streuli, M M, Krueger, N X NX, Thai, T T, Tang, M M and Saito, H H.. (1990) Distinct functional roles of the two intracellular phosphatase like domains of the receptor-linked protein tyrosine phosphatases LCA and LAR.. The EMBO journal, [PMID:1695146]
27. Tsai, A Y AY, Streuli, M M and Saito, H H.. (1989) Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.. Molecular and cellular biology, [PMID:2531281]
28. Streuli, M M, Hall, L R LR, Saga, Y Y, Schlossman, S F SF and Saito, H H.. (1987) Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.. The Journal of experimental medicine, (1): [PMID:2824653]
29. Charbonneau, H H, Tonks, N K NK, Walsh, K A KA and Fischer, E H EH.. (1988) The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2845400]
30. Ralph, S J SJ, Thomas, M L ML, Morton, C C CC and Trowbridge, I S IS.. (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen).. The EMBO journal, [PMID:2956090]
31. Hall, L R LR, Streuli, M M, Schlossman, S F SF and Saito, H H.. (1988) Complete exon-intron organization of the human leukocyte common antigen (CD45) gene.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2971730]
32. Kung, C C and 12 more authors.. (2000) Mutations in the tyrosine phosphatase CD45 gene in a child with severe combined immunodeficiency disease.. Nature medicine, [PMID:10700239]
33. Jacobsen, M M and 15 more authors.. (2000) A point mutation in PTPRC is associated with the development of multiple sclerosis.. Nature genetics, [PMID:11101853]
34. Tchilian, E Z EZ and 5 more authors.. (2001) A deletion in the gene encoding the CD45 antigen in a patient with SCID.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11145714]
35. Wu, Liangtang L, Fu, Jun J and Shen, Shi-Hsiang SH.. (2002) SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription.. Molecular and cellular biology, [PMID:11909961]
36. Fukuhara, Kenjiro K and 6 more authors.. (2002) A study on CD45 isoform expression during T-cell development and selection events in the human thymus.. Human immunology, [PMID:11975983]
37. Nam, Hyun-Joo HJ, Poy, Florence F, Saito, Haruo H and Frederick, Christin A CA.. (2005) Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45.. The Journal of experimental medicine, (7): [PMID:15684325]
38. Panchal, Rekha G RG and 10 more authors.. (2009) Reduced expression of CD45 protein-tyrosine phosphatase provides protection against anthrax pathogenesis.. The Journal of biological chemistry, (8): [PMID:19269962]
39. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
40. Gabaev, Ildar I and 9 more authors.. (2011) The human cytomegalovirus UL11 protein interacts with the receptor tyrosine phosphatase CD45, resulting in functional paralysis of T cells.. PLoS pathogens, [PMID:22174689]
41. Roberts, Joseph L JL and 12 more authors.. (2012) CD45-deficient severe combined immunodeficiency caused by uniparental disomy.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:22689986]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD45RA Mouse mAb (FITC)

库存信息

库存信息

库存信息

基本信息

产品属性

图片

关联靶点（人）

作用机制

安全和危险性（GHS）

质量标准

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

可替换产品

参考文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器