基本信息

产品名称	I-A/I-E Rat mAb (PE-Cy7)
别名	组织相容性复合体β（HLA-DMB）抗体 \| I-A/I-E抗体
英文别名	IAb \| Ia2 \| Ia-2 \| Abeta \| H-2Ab \| H2-Ab \| Rmcs1 \| I-Abeta \| Eb \| Ia4 \| H2Eb \| Ia-4 \| H-2Eb \| E-alpha-f \| H-2 class II histocompatibility antigen, E-K alpha chain \| H-2 class II histocompatibility antigen, E-U alpha chain \| H-2E \| H2-Ea \| H-2Ea \| H2-Ea-ps
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
宿主种属	大鼠(Rat)
特异性	I-A/I-E
种属反应性	小鼠(Mouse)
免疫原	Activated C57BL/6 mouse spleen cells
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	PE-Cy7
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	774nm
Ex/Em(nm)	Ex:565nm;Em:774nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: I-A/I-E Rat mAb (PE-Cy7) (Ab190260)

-A/I-E Rat mAb (PE-Cy7) (Ab190260) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with I-A/I-E Rat mAb (PE-Cy7) (Ab190260) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Flow Cytometry: I-A/I-E Rat mAb (PE-Cy7) (Ab190260)

I-A/I-E Rat mAb (PE-Cy7) (Ab190260) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with I-A/I-E Rat mAb (PE-Cy7) (Ab190260) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

HLA-DQB1 Tbio HLA II 类组织相容性抗原，DQ β 1 链(HLA class II histocompatibility antigen, DQ beta 1 chain) (0 活性数据)

点击查看靶蛋白信息： HLA-DQB1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

应用

应用名称	稀释比例
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.