基本信息

产品名称	CD31 Rat mAb (PE-Cy5)
别名	血小板内皮细胞粘附分子1(PECAM1)抗体 \| CD31抗体
英文别名	Cd31 \| Pecam \| adhesion molecule \| CD31 antigen \| CD31/EndoCAM \| EndoCAM \| FLJ34100 \| FLJ58394 \| GPIIA' \| PECA1 \| PECAM1 \| PECAM-1 \| PECAM-1, CD31/EndoCAM \| platelet endothelial cell adhesion molecule \| platelet endothelial cell adhesion molecule-1 \| plat
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
宿主种属	大鼠(Rat)
特异性	CD31
种属反应性	小鼠(Mouse)
免疫原	C3H/HeJ mouse hematopoietic progenitor cell line 3
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	PE-Cy5
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	670nm
Ex/Em(nm)	Ex:565nm;Em:670nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: CD31 Rat mAb (PE-Cy5) (Ab190234)

CD31 Rat mAb (PE-Cy5) (Ab190234) - Flow Cytometry
Flow cytometry analysis of CD31 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD31 Rat mAb (PE-Cy5) (Ab190234) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy5) (Ab190072).

Flow Cytometry: CD31 Rat mAb (PE-Cy5) (Ab190234)

CD31 Rat mAb (PE-Cy5) (Ab190234) - Flow Cytometry
Flow cytometry analysis of CD31 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD31 Rat mAb (PE-Cy5) (Ab190234) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

PECAM1 Tbio 血小板内皮细胞粘附分子(Platelet endothelial cell adhesion molecule) (0 活性数据)

点击查看靶蛋白信息： PECAM1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

应用

应用名称	稀释比例
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.