基本信息

产品名称	Caspase 3 Mouse mAb
别名	胱天蛋白酶3 \| 胱冬酶3 \| 胱天蛋白酶3抗体 \| CPP32抗体 \| CASP3抗体 \| 小鼠抗人胱冬酶3抗体 \| 胱冬酶3小鼠单克隆抗体 \| 胱冬酶3抗体 \| 小鼠抗人胱天蛋白酶3抗体 \| 胱天蛋白酶3小鼠单克隆抗体
英文别名	A830040C14Rik antibody \| Apopain antibody \| CASP 3 antibody \| CASP-3 antibody \| CASP3 antibody \| CASP3_HUMAN antibody \| Casp3a antibody \| Caspase 3 antibody \| Caspase 3 \| Caspase-3 subunit p12 antibody \| Caspase3 antibody \| CC3 antibody \| CPP 32 antibody
规格或纯度	ExactAb™, 已验证, 无载体, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively). Cleaves and inactivates interleukin-18 (IL18). Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC.
宿主种属	小鼠(Mouse)
特异性	Human CASP3
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属	Pan paniscus
免疫原	Recombinant protein corresponding to Human CASP3
阳性对照	WB: Jurkat, HeLa, HEK293, NIH/3T3 and C6 whole cell lysates; Mouse liver tissue lysates Flow Cytometry: Jurkat cells IF/ICC: HeLa cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

图片

Western Blot: Caspase 3 Mouse mAb (Ab156544)

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34,36 kDa
Exposure time: 16.2 s

Western Blot: Caspase 3 Mouse mAb (Ab156544)

Flow Cytometry: Caspase 3 Mouse mAb (Ab156544)

Caspase 3 Mouse mAb (Ab156544) - Flow Cytometry 
Flow Cytometry analysis of Jurkat cells stained with Caspase 3 Mouse mAb (Ab156544) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue).

ICC/IF: Caspase 3 Mouse mAb (Ab156544)

Caspase 3 Mouse mAb (Ab156544) - ICC/IF
IF analysis of Caspase 3 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caspase 3 Mouse mAb (Ab156544) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.