CEBPA Mouse mAb

    应用:
  • ELISA
  • Flow Cytometry
  • WB
功能和特点
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: 4E10
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab095961-10μl
10μl 现货 Stock Image
Ab095961-50μl
50μL 现货 Stock Image
Ab095961-100μl
100μL 现货 Stock Image
Ab095961-1ml
1ml 期货 Stock Image

基本信息

产品名称 CEBPA Mouse mAb
别名 CEBPA 小鼠单克隆抗体 | CEBPA 小鼠单抗
英文别名 C/EBP alpha
规格或纯度 ExactAb™, Validated, Carrier Free, 1.0mg/mL
宿主种属 小鼠(Mouse)
特异性 CEBPA
种属反应性 人(Human)
免疫原 Purified recombinant fragment of human CEBPA expressed in E. Coli.
阳性对照 WB: THP-1 cell lysate, Flow: HeLa cells.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 4E10
Format Whole IgG
亚型 IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 Purified antibody in PBS with 0.05% sodium azide
防腐剂 0.05% sodium azide
浓度 1.0mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 下运输。在 +4°C 下短期储存(1-2 周)。收货后建议分装。在 -20°C 下保存。避免冷冻/解冻循环。
分子类型 抗体

图片

CEBPA Mouse mAb (Ab095961) - Western Blot
All lanes: CEBPA Mouse mAb (Ab095961) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42&26 kDa
Observed band size: 42&26 kDa

CEBPA Mouse mAb (Ab095961) - Flow Cytometry
Flow cytometric analysis of HeLa cells using CEBPA Mouse mAb (Ab095961) at 1/200 dilution (green) and negative control (red).

关联靶点(人)

CEBPA Tbio CCAAT/增强子结合蛋白α(CCAAT/enhancer-binding protein alpha) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

安全和危险性(GHS)

质量标准

应用

应用名称 稀释比例
WB

1/500-1/2000

Flow Cytometry

1/200-1/400

ELISA

1/10000

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F1012042 分析证书 24-10-14 Ab095961
ZJ23F0700733 分析证书 23-08-01 Ab095961
ZJ23F0700732 分析证书 23-08-01 Ab095961

参考文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Antonson, P P and Xanthopoulos, K G KG..  (1995)  Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha)..  Biochemical and biophysical research communications,  (4): [PMID:7575576]
3. Choi, B H BH, Park, G T GT and Rho, H M HM..  (1999)  Interaction of hepatitis B viral X protein and CCAAT/ enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter..  The Journal of biological chemistry,  (29): [PMID:9915821]
4. Aho, S S and 7 more authors..  (2000)  Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors..  The Journal of cell biology,  (20): [PMID:10725330]
5. Pabst, T T and 8 more authors..  (2001)  Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute myeloid leukemia..  Nature genetics,  [PMID:11242107]
6. Snaddon, Jennifer J and 9 more authors..  (2003)  Mutations of CEBPA in acute myeloid leukemia FAB types M1 and M2..  Genes, chromosomes & cancer,  [PMID:12661007]
7. Müller, Christine C, Calkhoven, Cornelis F CF, Sha, Xiaojing X and Leutz, Achim A..  (2004)  The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest..  The Journal of biological chemistry,  (20): [PMID:14660596]
8. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,  (1): [PMID:15057824]
9. Wu, Frederick Y FY and 5 more authors..  (2004)  CCAAT/enhancer binding protein alpha binds to the Epstein-Barr virus (EBV) ZTA protein through oligomeric interactions and contributes to cooperative transcriptional activation of the ZTA promoter through direct binding to the ZII and ZIIIB motifs during induction of the EBV lytic cycle..  Journal of virology,  [PMID:15078966]
10. Wang, Guo-Li GL, Iakova, Polina P, Wilde, Margie M, Awad, Samir S and Timchenko, Nikolai A NA..  (2004)  Liver tumors escape negative control of proliferation via PI3K/Akt-mediated block of C/EBP alpha growth inhibitory activity..  Genes & development,  (15): [PMID:15107404]
11. Smith, Matthew L ML, Cavenagh, Jamie D JD, Lister, T Andrew TA and Fitzgibbon, Jude J..  (2004)  Mutation of CEBPA in familial acute myeloid leukemia..  The New England journal of medicine,  (2): [PMID:15575056]
12. Müller, Christine C, Bremer, Anna A, Schreiber, Sandra S, Eichwald, Sabrina S and Calkhoven, Cornelis F CF..  (2010)  Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size..  The EMBO journal,  (3): [PMID:20075868]
13. Zaragoza, Katrin K, Bégay, Valérie V, Schuetz, Anja A, Heinemann, Udo U and Leutz, Achim A..  (2010)  Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes..  Molecular and cellular biology,  [PMID:20176812]
14. Dedhia, Priya H PH and 9 more authors..  (2010)  Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia..  Blood,  (26): [PMID:20410507]
15. Uljon, Sacha and 7 more authors..  (2016)  Structural Basis for Substrate Selectivity of the E3 Ligase COP1..  Structure (London, England : 1993),  (3): [PMID:27041596]
16. Brunmeir, Reinhard R and 7 more authors..  (2016)  Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis..  PLoS genetics,  [PMID:27923061]

溶液计算器