TRIS醋酸盐-EDTA缓冲液

适用于电泳

BioReagent

MDL号: MFCD00236357

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
T755532-1L	1L	期货

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基本描述

规格或纯度	BioReagent, 用于电泳
英文名称	Tris Acetate-EDTA buffer
储存温度	室温
运输条件	常规运输
产品介绍	Tris-乙酸缓冲液EDTA缓冲液作为运行缓冲液，用于人类乳头状瘤病毒DNA的琼脂糖凝胶电泳以及转录的RNA样品。TAE流动缓冲液是DNA琼脂糖凝胶电泳最常用的缓冲液，也可用于非变性RNA琼脂糖凝胶电泳。双链DNA在TAE中比在其他缓冲液中运行更快，但也可能在延长的电泳过程中耗尽。在延长的电泳过程中，缓冲液循环或缓冲液更换可以弥补较低的缓冲容量。浓缩TAE缓冲液稀释后得到1× TAE缓冲液，加入40 mM醋酸三酯和1 mM EDTA，pH 8.3。 1× TAE缓冲液用于琼脂糖凝胶和作为流动缓冲液。为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。 Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples. TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.