重组粘性链霉亲和素(r-ScSA)

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货号 (SKU) 包装规格 是否现货 价格 数量
S767462-1mg
1mg 期货 Stock Image
S767462-5mg
5mg 期货 Stock Image
S767462-20mg
20mg 现货 Stock Image
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SA (46) 链霉亲和素 (51)

基本描述

别名 粘性重组链霉亲和素 | 链霉亲和素
英文别名 Sticky Recombinant Streptavidin | r-ScSA | Streptavidin | SA
规格或纯度 BioReagent, 用于蛋白分析, for ELISA, 适用于免疫组织化学, ≥95%(SDS-PAGE), ≥ 14 U/mg
稳定性与储存 Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
英文名称 Recombinant Sticky Streptavidin (r-ScSA)
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
产品介绍

分子量:54-94 kDa

物理外观:Lyophilized

储存缓冲液:Lyophilized from 5mM PBS, pH 7.4

复溶(Reconstitution):Reconstitute in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

产品描述:

  链霉亲和素(Streptavidin,SA)是阿维丁链霉菌(Streptomyces avidinii)在生长过程中分泌的一种同型四聚体蛋白。同亲和素一样,一摩尔的链霉亲和素可以结合四摩尔的生物素,与生物素具有很高的亲和力。由于 SA 不含糖基,因此 SA 在检测应用中具有比亲和素更低的非特异性结合水平,已被广泛应用于酶联免疫吸附实验、免疫组织化学、时间分辨免疫荧光技术(TRFIA)、定量 PCR、单链 DNA 制备、生物分子纯化、单克隆抗体制备等生物技术领域。
  但是链霉亲和素的吸附性较差,在实际应用中尤其是吸附到 NC 膜或包埋比较困难,粘性重组链霉亲和素(S-rSA)主要是解决这个问题,其不仅具有与活性有关的核心序列,而且吸附性、稳定性、溶解性等方面均优于天然 SA,尤其在 NC 膜上的吸附性远远超越天然 SA。


Slot-Blot 法 NC 膜上不同 SA 吸附及活性比较
注意:本产品仅限于科研使用。  

阿拉丁链霉亲和素系列产品:

货号产品名称物理外观储存缓冲液分子量来源活性(U/mg蛋白)关键特性
S274235重组链霉亲和素 (r-SA)LyophilizedLyophilized from 5mM PBS, pH 6.062.4kDa (tetramer), 16.5kDa (monomer)E.coli≥15C端含His-Tag;pI=6.42
R748131重组单体链霉亲和素(r-mSA)LyophilizedLyophilized from 10mM CBS, Trehalose, pH 9.215.1kDaE.coli≥8N-His tag,pI=7.1,易洗脱
S767455重组增强稳定型核心链霉亲和素(se-cSA)LyophilizedLyophilized from 5mM PPB, Trehalose, pH 7.453.6kDa (tetramer), 13.4kDa (monomer)E.coli17-19核心序列优化,序列修饰,热稳定性、溶解性、活性等提升;pI=5.68
S767462重组粘性链霉亲和素 (r-ScSA)LyophilizedLyophilized from 5mM PBS, pH 7.454-94kDaE.coli≥14增强吸附性(特别适用于NC膜),保留核心活性
R767460重组核心链霉亲和素 (r-cSA)LyophilizedLyophilized from 5mM PBS, pH 7.454kDa (tetramer), 13.5kDa (monomer)E.coli≥15核心序列优化;C端含半胱氨酸;pI=6.04
R767461重组核心链霉亲和素2 (r-cSA2)LyophilizedLyophilized from 5mM PBS, pH 7.453.2kDa (tetramer), 13.3kDa (monomer)E.coli≥16核心序列优化;pI=6.09
R767452重组核心链霉亲和素4 (r-cSA4)LyophilizedLyophilized from 5mM PPB, Trehalose, pH 7.455.2kDa (tetramer), 13.8kDa (monomer)E.coli≥16核心序列优化,序列修饰(增加了氨基的含量),稳定性、溶解性等提升;pI=8.93
R767450重组长臂巯基核心链霉亲和素LyophilizedLyophilized from 20mM HEPES, Trehalose, TCEP, pH 7.054.8kDa (tetramer), 13.7kDa (monomer)E.coli≥16核心序列优化,C端的半胱氨酸前添加6个柔性氨基酸延长巯基臂;pI=6.09

Molecular weight: 54-94 kDa

Physical appearance: Lyophilized

Buffer storage: Lyophilized from 5mM PBS, pH 7.4

Reconstitution:Reconstitute in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Product introduction:

Streptavidin (SA) is a homologous tetramer protein secreted by Streptomyces avidinii during its growth. As with avidin, one mole of streptavidin can bind four moles of biotin, and biotin has a high affinity. Because SA does not contain a sugar group, SA has a lower non-specific binding level than avidin in detection applications. It has been widely used in enzyme-linked immunosorbent assay, immunohistochemistry, time-resolved immunofluorescence technique (TRFIA), quantitative PCR, single-strand DNA preparation, biomolecular purification, monoclonal antibody preparation and other biotechnology fields.
However, the adsorbability of streptavidin is poor, and it is difficult to adsorb or embed it into NC film in practical applications. Viscous recombinant streptavidin (S-rSA) is mainly used to solve this problem. It not only has the core sequence related to activity, but also has better adsorption, stability and solubility than natural SA. In particular, the adsorption of NC film is far more than that of natural SA.


Comparison of adsorption and activity of different SA on NC films by Slot-Blot method 

Note: This product is for scientific use only. 


Source 

E. coli

名称和识别符

分子类型 蛋白质

安全和危险性(GHS)

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批号(Lot Number) 证书类型 日期 货号
ZJ25F0521494 分析证书 25-05-15 S767462

溶液计算器