基本描述

产品名称	链霉亲合素
别名	链亲合素
英文别名	EX-A4899 \| CID 51062757 \| FT-0700692 \| 2-[(3-chlorophenyl)-piperidin-1-ium-1-ylidenemethyl]sulfanylacetic acid;bromide
规格或纯度	from Streptomyces avidinii,≥12 U/mg protein
生化机理	链霉亲和素是一种生物素结合蛋白，最初是从阿维丁链霉菌（Streptomyces avidinii）中分离出来的。与阿维丁不同，链霉亲和素没有碳水化合物，pI 值为 5，呈弱酸性。我们的链霉亲和素产品使用的是重组形式的链霉亲和素，其质量为 53,000 道尔顿，pI 值接近中性。链霉亲和素是一种四聚体蛋白，每个亚基与一分子生物素结合，亲和力与阿维丁相似。氯化胍会将阿维丁和链霉亲和素解离成亚基，但链霉亲和素更耐解离。
产品介绍	Streptavidin and Conjμgates include streptavidin in a purified form, conjμgated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjμgated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjμgates (peroxidase-conjμgated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjμgates are supplied as a liquid in a Tris-based buffer. Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting) Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting)
偶联	Unconjugated

产品规格参数

物理形态	固体
浓度	from Streptomyces avidinii,≥12 U/mg protein
储存温度	-20°C储存,充氩
运输条件	超低温冰袋运输
CAS编号和信息	9013-20-1

安全和危险性（GHS）

象形图	GHS07
信号词	Warning
危险声明	H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H335: 可能引起呼吸道刺激
预防措施声明	P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P405: 密闭存放 P501: 将内容物/容器处理到。。。 P264: 处理后要彻底洗手。 P271: 仅在室外或通风良好的地方使用。 P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。 P403+P233: 存放在通风良好的地方。保持容器密闭。 P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。 P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。 P337+P317: 如果眼睛刺激持续：寻求医疗帮助。 P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。 P319: 如果你感到不适，请寻求医疗帮助。
WGK Germany	3
个人防护装备	Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

SDS英文版

质量标准

Activity	≥12（units/mg）
Appearance（S103034)	white to light brown powder or crystals
DNases	none detected
Electrophoresis test	pass
proteases	none detected
RNases	none detected
Solubility in H2O，Colorless to Yellow Clear to Hazy 1mg vial/ml	pass

质量标准

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批号(Lot Number)	证书类型	日期	货号
C2124257	分析证书	25-01-10	S103034
K2429743	分析证书	24-12-12	S103034
K2429752	分析证书	24-12-12	S103034
H2407459	分析证书	24-07-26	S103034
H2407457	分析证书	24-07-26	S103034
H2407460	分析证书	24-07-26	S103034
H2407461	分析证书	24-07-26	S103034
H2407458	分析证书	24-07-26	S103034
K2204620	分析证书	22-09-24	S103034
K2204622	分析证书	22-09-24	S103034
K2204634	分析证书	22-09-24	S103034
K2204635	分析证书	22-09-24	S103034
K2204636	分析证书	22-09-24	S103034
K2204637	分析证书	22-09-24	S103034
J2330032	分析证书	22-09-24	S103034
I2405016	分析证书	22-09-24	S103034
D2410080	分析证书	22-06-12	S103034
F2221393	分析证书	22-06-12	S103034
F2221397	分析证书	22-06-12	S103034
F2221398	分析证书	22-06-12	S103034
F2221401	分析证书	22-06-12	S103034
F2221514	分析证书	22-06-12	S103034
F2221515	分析证书	22-06-12	S103034
G2229096	分析证书	22-06-12	S103034

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参考文献

1. Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang. (2024) CRISPR/Cas12a-powered CLASA towards OTA ultrasensitive detection in cereal samples. MICROCHEMICAL JOURNAL, 196 (109691). [10.1016/j.microc.2023.109691]
2. Huang Lianrun, Wang Guixiu, Wu Yanling, Wang Zhijia, Ding Yuan, Liang Hongwu, Hua Xiude. (2024) Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides. MICROCHIMICA ACTA, 191 (1): (1-11). [PMID:38159155] [10.1007/s00604-023-06151-w]
3. Yuxiu Fan, Dong Li, Xiaoyi Xie, Yi Zhang, Ling Jiang, Bin Huang, Xiupei Yang. (2024) Flower-like L-Cys-FeNiNPs nanozyme aptasensor for sensitive colorimetric detection of aflatoxin B1. MICROCHEMICAL JOURNAL, 197 (109842). [10.1016/j.microc.2023.109842]
4. Yuan Shun, Che Yanjia, Wang Zhiwei, Xing Kai, Xie Xiaoping, Chen Yuanyang. (2023) Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia‒reperfusion injury in heart transplantation. Journal of Translational Medicine, 21 (1): (1-23). [PMID:38124174] [10.1186/s12967-023-04763-7]
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26. Zhaode Mu, Jiangman Tian, Jie Wang, Jing Zhou, Lijuan Bai. (2022) A new electrochemical aptasensor for ultrasensitive detection of endotoxin using Fe-MOF and AgNPs decorated P-N-CNTs as signal enhanced indicator. APPLIED SURFACE SCIENCE, 573 (151601). [10.1016/j.apsusc.2021.151601]
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