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蛋白质
链霉亲和素偶联物
Recombinant Streptavidin Protein (Oregon Green 488)

Recombinant Streptavidin Protein (Oregon Green 488)

已验证

ExactAb™

1.0 mg/mL

功能和特点

表达系统: E. coli
生物活性: Binding Biotin

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp217313-10μg	10μg	期货
rp217313-100μg	100μg	期货
rp217313-1mg	1mg	期货

查看相关系列

Oregon Green 488 (3) SA (46) Streptavidin Protein (25) 链霉亲和素 (51)

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基本描述

产品名称	Recombinant Streptavidin Protein (Oregon Green 488)
别名	重组链霉亲和素 \| 链霉亲和素
英文别名	SA V1 \| SA V2 \| strepavidin \| Streptavidin V1 \| Streptavidin V2 \| Streptavidin
规格或纯度	ExactAb™, 已验证, 1.0 mg/mL
生化机理	链霉亲和素是一种生物素结合蛋白，能结合四种生物素分子。链霉亲和素的生物素结合口袋有助于与生物素相互作用。它可以用来增强蛋白质结合和多聚化。
产品介绍	Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.
生物活性	Binding Biotin
表达系统	E. coli
无载体	No
无动物源	No
偶联	Oregon Green 488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	526nm
Ex/Em(nm)
来源	重组表达

图片

IF: Recombinant Streptavidin Protein (Oregon Green 488) (rp217313)

Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (Oregon Green 488) (rp217313)

Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) - Flow Cytometry
Flow cytometry analysis of EpCAM expression by HT-29 cells (red) compared to an isotype control (blue). Cells were stained with biotinylated Oportuzumab (anti-EpCAM) (Ab190146) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant Streptavidin Protein (Oregon Green 488) (rp217313)

Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

产品规格参数

物理形态	液体
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300
防腐剂	0.05% ProClin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
CAS编号和信息	9013-20-1
分子类型	蛋白质

安全和危险性（GHS）

象形图	GHS07
信号词	Warning
危险声明	H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H335: 可能引起呼吸道刺激
预防措施声明	P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P405: 密闭存放 P501: 将内容物/容器处理到。。。 P264: 处理后要彻底洗手。 P271: 仅在室外或通风良好的地方使用。 P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。 P403+P233: 存放在通风良好的地方。保持容器密闭。 P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。 P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。 P337+P317: 如果眼睛刺激持续：寻求医疗帮助。 P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。 P319: 如果你感到不适，请寻求医疗帮助。
WGK Germany	3
个人防护装备	Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

SDS英文版

应用

应用名称	稀释比例
IF/ICC	1-10μg/mL
Flow Cytometry	Flow Cytometry: 0.5-10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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批号(Lot Number)	证书类型	日期	货号
ZJ25R0402331	分析证书	25-04-13	rp217313

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Recombinant Streptavidin Protein (Oregon Green 488)

库存信息

库存信息

库存信息

基本描述

图片

产品规格参数

安全和危险性（GHS）

质量标准

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器