Recombinant Human Vimentin Protein

COA

级别和纯度:

无载体

无叠氮钠

≥90%(SDS-PAGE)

表达系统: E. coli
Accession #: P08670
蛋白标签: C-His
生物活性: Testing in progress

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp156639-10μg	10μg	现货
rp156639-50μg	50μg	现货
rp156639-100μg	100μg	期货
rp156639-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: VIM

Accession#:P08670 (5) Gene ID:7431 (5) VIM (5) Vimentin (5)

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基本描述

产品名称	Recombinant Human Vimentin Protein
别名	重组人Vimentin蛋白 \| 重组人类波形蛋白
英文别名	FLJ36605 \| VIM \| Vimentin \| epididymis secretory sperm binding protein \| CTRCT30 \| Epididymis luminal protein 113 \| HEL113 \| VIME_HUMAN
规格或纯度	无载体, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The Vimentin protein is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. Vimentin is the predominant intermediate filament in cells of mesenchymal origin such as vascular endothelium and blood cells. The human Vimentin cDNA encodes a 466 amino acid (aa) Vimentin protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers. The human Vimentin protein shares 97-98% aa identity with the mouse, rat, bovine and canine Vimentin proteins. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments. These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress. Intermediate filament fibers are highly dynamic, and their half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives intermediate filament disintegration, followed by reorganization during mitosis. Interactions of head and tail domains link intermediate filaments with other structures such as actin and microtubule cytoskeletons. The Vimentin protein is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge. Vimentin also helps maintain the lipid composition of cellular membranes, and caspase cleavage of the Vimentin protein is a key event in apoptosis. Phosphorylation of the Vimentin protein promotes its secretion by TNF-alpha -stimulated macrophages. Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst. Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes.
生物活性	Testing in progress
表达系统	E. coli
种属	人(Human)
氨基酸	1- 466 aa
序列	MGMSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLEHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	No
Accession #	P08670
来源	重组表达
预测分子量	54.6 kDa
SDS-PAGE	54.1 kDa, reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM Tris, 2M Urea, 0.1M NaH2PO4, pH6.3
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Vimentin protein (rp156639)

Recombinant Human Vimentin protein (rp156639) - SDS-PAGE
Recombinant Human Vimentin protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 54.1 kDa.

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找到2个结果

批号(Lot Number)	证书类型	日期	货号
ZJ23F1101766	分析证书	25-06-24	rp156639
ZJ23F1101767	分析证书	25-06-24	rp156639

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Recombinant Human Vimentin Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

此产品的引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器