货号 (SKU)

包装规格

是否现货

价格

数量

rp156635-10μg

10μg

现货

rp156635-100μg

100μg

现货

rp156635-1mg

1mg

期货

基本描述

产品名称	Recombinant Human Histone H3 Protein
别名	重组人类组蛋白H3蛋白
英文别名	Histone H3/a \| Histone H3/b \| Histone H3/c \| Histone H3/d \| Histone H3/f \| Histone H3/h \| Histone H3/i \| Histone H3/j \| Histone H3/k \| Histone H3/l
规格或纯度	Carrier Free, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 histone family member E pseudogene, H3 histone family, member A, H3/A, H31_HUMAN, H3F3, H3FA, Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, HIST3H3, histone 1, H3a, Histone cluster 1, H3a
表达系统	E. coli
种属	人(Human)
氨基酸	1-136 aa
序列	MGHHHHHHMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
纯度	>90% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P68431
来源	重组表达
预测分子量	15.4 kDa
SDS-PAGE	16.2 kDa, reducing conditions; 16.2 kDa, non-reducing conditions

图片

SDS-PAGE: Recombinant Human Histone H3 Protein (rp156635)

Recombinant Human Histone H3 Protein (rp156635) - SDS-PAGE
Recombinant Human Histone H3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.4 kDa under reducing conditions, showing two bands at 16.2 kDa and 32.0 kDa under non-reducing conditions .

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 2M Urea, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

安全和危险性（GHS）

SDS英文版

质量标准

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	日期	货号
ZJ25R0402352	分析证书	25-04-18	rp156635
ZJ23F1101867	分析证书	23-12-13	rp156635

参考文献

1. Ananthanarayanan M, Li S, Balasubramaniyan N, Suchy FJ, Walsh MJ. (2004) Ligand-dependent activation of the farnesoid X-receptor directs arginine methylation of histone H3 by CARM1.. J Biol Chem, 279 (52): (54348-57). [PMID:15471871]
2. Marashi, F F and 6 more authors.. (1986) Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5' regulatory sequences.. Biochemistry and cell biology = Biochimie et biologie cellulaire, [PMID:3013246]
3. Zhong, R R, Roeder, R G RG and Heintz, N N.. (1983) The primary structure and expression of four cloned human histone genes.. Nucleic acids research, (11): [PMID:6647026]
4. Ohe, Y Y and Iwai, K K.. (1981) Human spleen histone H3. Isolation and amino acid sequence.. Journal of biochemistry, [PMID:7309716]
5. Goto, H H and 10 more authors.. (1999) Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation.. The Journal of biological chemistry, (3): [PMID:10464286]
6. Lachner, M M, O'Carroll, D D, Rea, S S, Mechtler, K K and Jenuwein, T T.. (2001) Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.. Nature, (1): [PMID:11242053]
7. Preuss, Ute U, Landsberg, Gerd G and Scheidtmann, Karl Heinz KH.. (2003) Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase.. Nucleic acids research, (1): [PMID:12560483]
8. Huyen, Yentram Y and 9 more authors.. (2004) Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks.. Nature, (18): [PMID:15525939]
9. Dai, Jun J, Sultan, Sammy S, Taylor, Stephen S SS and Higgins, Jonathan M G JM.. (2005) The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.. Genes & development, (15): [PMID:15681610]
10. Choi, Hong Seok HS and 5 more authors.. (2005) Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha.. The Journal of biological chemistry, (8): [PMID:15684425]
11. Macdonald, Neil N and 12 more authors.. (2005) Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3.. Molecular cell, (28): [PMID:16246723]
12. Hake, Sandra B SB and 8 more authors.. (2006) Expression patterns and post-translational modifications associated with mammalian histone H3 variants.. The Journal of biological chemistry, (6): [PMID:16267050]
13. Flanagan, John F JF and 8 more authors.. (2005) Double chromodomains cooperate to recognize the methylated histone H3 tail.. Nature, (22): [PMID:16372014]
14. Thomas, C Eric CE, Kelleher, Neil L NL and Mizzen, Craig A CA.. (2006) Mass spectrometric characterization of human histone H3: a bird's eye view.. Journal of proteome research, [PMID:16457588]
15. Miao, Feng F, Li, ShuLian S, Chavez, Valerie V, Lanting, Linda L and Natarajan, Rama R.. (2006) Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.. Molecular endocrinology (Baltimore, Md.), [PMID:16497732]
16. Beck, Hans Christian HC and 8 more authors.. (2006) Quantitative proteomic analysis of post-translational modifications of human histones.. Molecular & cellular proteomics : MCP, [PMID:16627869]
17. Morris, Stephanie A SA and 9 more authors.. (2007) Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification.. The Journal of biological chemistry, (9): [PMID:17189264]
18. Garcia, Benjamin A BA and 10 more authors.. (2007) Organismal differences in post-translational modifications in histones H3 and H4.. The Journal of biological chemistry, (9): [PMID:17194708]
19. Guccione, Ernesto E and 7 more authors.. (2007) Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive.. Nature, (18): [PMID:17898714]
20. Metzger, Eric E and 11 more authors.. (2008) Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation.. Nature cell biology, [PMID:18066052]
21. Iberg, Aimee N AN and 6 more authors.. (2008) Arginine methylation of the histone H3 tail impedes effector binding.. The Journal of biological chemistry, (8): [PMID:18077460]
22. Hyllus, Dawin D and 7 more authors.. (2007) PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.. Genes & development, (15): [PMID:18079182]
23. Shimada, Midori M and 6 more authors.. (2008) Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression.. Cell, (25): [PMID:18243098]
24. Metzger, Eric E and 14 more authors.. (2010) Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4.. Nature, (1): [PMID:20228790]
25. Vermeulen, Michiel M and 10 more authors.. (2010) Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.. Cell, (17): [PMID:20850016]
26. Wu, Gang and 21 more authors.. (2012) Somatic histone H3 alterations in pediatric diffuse intrinsic pontine gliomas and non-brainstem glioblastomas.. Nature genetics, (29): [PMID:22286216]
27. Yu, Yongxin Y and 7 more authors.. (2012) Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA.. Molecular cell, (13): [PMID:22387026]
28. Yang, Weiwei W and 9 more authors.. (2012) PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis.. Cell, (17): [PMID:22901803]
29. Tropberger, Philipp P and 11 more authors.. (2013) Regulation of transcription through acetylation of H3K122 on the lateral surface of the histone octamer.. Cell, (14): [PMID:23415232]
30. Chan, Kui-Ming KM and 11 more authors.. (2013) The histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expression.. Genes & development, (1): [PMID:23603901]
31. Lu, Chao C and 29 more authors.. (2016) Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape.. Science (New York, N.Y.), (13): [PMID:27174990]
32. Li, Lei L and 14 more authors.. (2016) SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability.. Nature communications, (20): [PMID:27436229]
33. Herranz, Nicolás N and 12 more authors.. (2016) Lysyl oxidase-like 2 (LOXL2) oxidizes trimethylated lysine 4 in histone H3.. The FEBS journal, [PMID:27735137]
34. Papillon-Cavanagh, Simon S and 22 more authors.. (2017) Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas.. Nature genetics, [PMID:28067913]
35. Wang, Yugang Y and 17 more authors.. (2017) KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase.. Nature, (14): [PMID:29211711]
36. Palazzo, Luca L and 5 more authors.. (2018) Serine is the major residue for ADP-ribosylation upon DNA damage.. eLife, (26): [PMID:29480802]
37. Kardalinou, E E, Eick, S S, Albig, W W and Doenecke, D D.. (1993) Association of a human H1 histone gene with an H2A pseudogene and genes encoding H2B.1 and H3.1 histones.. Journal of cellular biochemistry, [PMID:8227173]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human Histone H3 Protein

库存信息

库存信息

库存信息

基本描述

图片

产品规格参数

安全和危险性（GHS）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

参考文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器