基本描述

产品名称	Recombinant Human IL-2 Protein
别名	重组人白细胞介素-2蛋白 \| 重组人 IL-2 蛋白
英文别名	IL-2 \| T-cell growth factor \| TCGF
规格或纯度	ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	白介素2即白细胞介素-2（interleukin-2，IL-2），又名T细胞生长因子(T cell growth factor，TCRF)。主要由活化的CD4+T细胞和CD8+T细胞产生的具有广泛生物活性的细胞因子。是所有T细胞亚群的生长因子，能使T细胞在试管内长期存活，并可促进活化B细胞增殖，故为调控免疫应答的重要因子，也参与抗体反应、造血和肿瘤监视。本产品由真核细胞表达的重组蛋白，是将蛋白基因接入稳定表达载体，转化CHO-K1细胞，经克隆筛选，质量验证，挑选的稳定表达株，由化学成分确定的培养基（不含有任何动物源成分）发酵制备，工艺稳定，保证了产品质量及批次间的一致性。本产品为无菌冻干粉剂，由含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干。 Purity ≥95% (SDS-PAGE, SEC-HPLC) Additional sequence information 133 amino acids Function Interleukin-2, or interleukin-2, is also known as T cell growth factor (TCRF). A wide range of bioactive cytokines produced primarily by activated CD4+T cells and CD8+T cells. It is a growth factor of all T cell subsets, which can make T cells survive in vitro for a long time and promote the proliferation of activated B cells. Therefore, it is an important factor in the regulation of immune response, and also involved in antibody response, hematopoietic and tumor surveillance. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. This product is a recombinant protein expressed by eukaryotic cells. The protein gene was inserted into stable expression vector and transformed into CHO-K1 cells. After cloning, screening and quality verification, the selected stable expression strain was prepared by fermentation of the medium determined by chemical composition (without any animal source components). This product is a sterile lyophilized powder, made from a protein solution containing 10mM phosphate with a pH of 7.2 filtered by 0.2μm.
生物活性	1、Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically less than 2.5 ng/mL. 2、Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	CHO-K1
种属	人(Human)
氨基酸	21-153 aa
序列	APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
纯度	≥95% (SDS-PAGE, SEC-HPLC)
蛋白标签	His
蛋白长度	Full length protein
无载体	Yes
Accession #	P60568
来源	重组表达
预测分子量	15.4 kDa
SDS-PAGE	13.0 kDa, under reducing conditions; 13.0 kDa, under non-reducing conditions.

图片

Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476)-Protein Bioactivity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <2.5ng/mL.

SDS-PAGE: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) -SDS-PAGE
3μg/lane of Recombinant Human IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.0 kDa.

Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) - Protein Bioactivity
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干
复溶	建议将冻干 rhIL-2溶解在注射用水或灭菌的超纯水中，浓度不低于100μg/ml，以待进一步稀释至工作浓度。尽可能避免反复冻融。复溶后的细胞因子：4℃可稳定储存7-10天；短期保存请分装后存放于-20℃。
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	发货时温度为 4°C。储存温度为 -20°C。冻干，无添加剂。本品为活性蛋白质，可能会引起体内生物反应，请谨慎处理。
分子类型	蛋白质

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找到7个结果

批号(Lot Number)	证书类型	日期	货号
ZJ24F1113698	分析证书	24-11-14	rp147476
ZJ24F1113697	分析证书	24-11-14	rp147476
ZJ24F1113623	分析证书	24-11-14	rp147476
ZJ24F1113622	分析证书	24-11-14	rp147476
ZJ23F0600484	分析证书	23-07-03	rp147476
ZJ23F0600486	分析证书	23-07-03	rp147476
ZJ23F0600485	分析证书	23-07-03	rp147476

参考文献

1. Quéméner A, Maillasson M, Arzel L, Sicard B, Vomiandry R, Mortier E, Dubreuil D, Jacques Y, Lebreton J, Mathé-Allainmat M. (2017) Discovery of a Small-Molecule Inhibitor of Interleukin 15: Pharmacophore-Based Virtual Screening and Hit Optimization.. J Med Chem, 60 (14): (6249-6272). [PMID:28657314]
2. Chen L, Du J, Dai Q, Zhang H, Pang W, Hu J.. (2014) Prediction of anti-tumor chemical probes of a traditional Chinese medicine formula by HPLC fingerprinting combined with molecular docking.. Eur J Med Chem, 83 (294-306). [PMID:24974349] [10.1016/j.ejmech.2014.06.037]
3. Żyżyńska-Granica B, Trzaskowski B, Niewieczerzał S, Filipek S, Zegrocka-Stendel O, Dutkiewicz M, Krzeczyński P, Kowalewska M, Koziak K.. (2017) Pharmacophore guided discovery of small-molecule interleukin 15 inhibitors.. Eur J Med Chem, 136 (543-547). [PMID:28535470] [10.1016/j.ejmech.2017.05.034]
4. Laâbi, Y Y and 6 more authors.. (1992) A new gene, BCM, on chromosome 16 is fused to the interleukin 2 gene by a t(4;16)(q26;p13) translocation in a malignant T cell lymphoma.. The EMBO journal, [PMID:1396583]
5. Mott, H R HR and 5 more authors.. (1992) Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.. Biochemistry, (25): [PMID:1510960]
6. and Bazan, J F JF.. (1992) Unraveling the structure of IL-2.. Science (New York, N.Y.), (17): [PMID:1631562]
7. Conradt, H S HS and 5 more authors.. (1989) Expression of human interleukin-2 in recombinant baby hamster kidney, Ltk-, and Chinese hamster ovary cells. Structure of O-linked carbohydrate chains and their location within the polypeptide.. The Journal of biological chemistry, (15): [PMID:2793860]
8. Weir, M P MP, Chaplin, M A MA, Wallace, D M DM, Dykes, C W CW and Hobden, A N AN.. (1988) Structure-activity relationships of recombinant human interleukin 2.. Biochemistry, (6): [PMID:3264184]
9. Siebenlist, U U and 7 more authors.. (1986) Promoter region of interleukin-2 gene undergoes chromatin structure changes and confers inducibility on chloramphenicol acetyltransferase gene during activation of T cells.. Molecular and cellular biology, [PMID:3491296]
10. Brandhuber, B J BJ, Boone, T T, Kenney, W C WC and McKay, D B DB.. (1987) Three-dimensional structure of interleukin-2.. Science (New York, N.Y.), (18): [PMID:3500515]
11. Devos, R R and 7 more authors.. (1983) Molecular cloning of human interleukin 2 cDNA and its expression in E. coli.. Nucleic acids research, (11): [PMID:6306584]
12. Maeda, S S and 9 more authors.. (1983) Cloning of interleukin 2 mRNAs from human tonsils.. Biochemical and biophysical research communications, (30): [PMID:6312994]
13. Fujita, T T, Takaoka, C C, Matsui, H H and Taniguchi, T T.. (1983) Structure of the human interleukin 2 gene.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6324170]
14. Holbrook, N J NJ, Lieber, M M and Crabtree, G R GR.. (1984) DNA sequence of the 5' flanking region of the human interleukin 2 gene: homologies with adult T-cell leukemia virus.. Nucleic acids research, (25): [PMID:6330695]
15. Robb, R J RJ, Kutny, R M RM, Panico, M M, Morris, H R HR and Chowdhry, V V.. (1984) Amino acid sequence and post-translational modification of human interleukin 2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6333684]
16. Taniguchi, T T and 6 more authors.. (1983) Structure and expression of a cloned cDNA for human interleukin-2.. Nature, [PMID:6403867]
17. Holbrook, N J NJ and 5 more authors.. (1984) T-cell growth factor: complete nucleotide sequence and organization of the gene in normal and malignant cells.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6608729]
18. Bamborough, P P, Hedgecock, C J CJ and Richards, W G WG.. (1994) The interleukin-2 and interleukin-4 receptors studied by molecular modelling.. Structure (London, England : 1993), (15): [PMID:7529123]
19. Eizenberg, O O, Faber-Elman, A A, Lotan, M M and Schwartz, M M.. (1995) Interleukin-2 transcripts in human and rodent brains: possible expression by astrocytes.. Journal of neurochemistry, [PMID:7722480]
20. Miyazaki, T T and 9 more authors.. (1994) Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits.. Science (New York, N.Y.), (11): [PMID:7973659]
21. Chernicky, C L CL, Tan, H H, Burfeind, P P, Ilan, J J and Ilan, J J.. (1996) Sequence of interleukin-2 isolated from human placental poly A+ RNA: possible role in maintenance of fetal allograft.. Molecular reproduction and development, [PMID:8824916]
22. Wang, Xinquan X, Rickert, Mathias M and Garcia, K Christopher KC.. (2005) Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors.. Science (New York, N.Y.), (18): [PMID:16293754]
23. Stauber, Deborah J DJ, Debler, Erik W EW, Horton, Patricia A PA, Smith, Kendall A KA and Wilson, Ian A IA.. (2006) Crystal structure of the IL-2 signaling complex: paradigm for a heterotrimeric cytokine receptor.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:16477002]
24. Fiebich, Bernd L BL and 6 more authors.. (2012) Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.. Immunopharmacology and immunotoxicology, [PMID:21631396]

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货号 (SKU)	包装规格	是否现货
rp147476-10μg	10μg	现货
rp147476-50μg	50μg	现货
rp147476-100μg	100μg	现货
rp147476-1mg	1mg	期货

Recombinant Human IL-2 Protein

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基本描述

图片

产品规格参数

安全和危险性（GHS）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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参考文献

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