Recombinant Human FABP4 Protein

无动物源

无载体

生物活性

ActiBioPure™

无叠氮钠

≥95%(SDS-PAGE)

功能和特点

表达系统: HEK293
Accession #: P15090
蛋白标签: N-His
生物活性: The binding activity of FABP4 with ACTB.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp145772-10μg	10μg	现货
rp145772-50μg	50μg	现货
rp145772-100μg	100μg	现货
rp145772-1mg	1mg	期货

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点击查看蛋白信息: FABP4

Accession#:P15090 (2) FABP4 (2) Gene ID:2167 (2)

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基本描述

产品名称	Recombinant Human FABP4 Protein
别名	重组人FABP4蛋白 \| 重组人 FABP4 蛋白
英文别名	3T3-L1 lipid-binding protein \| 422/aP2 \| A-FABP \| Adipocyte \| Adipocyte lipid binding protein \| Adipocyte lipid-binding protein \| Adipocyte protein AP2 \| Adipocyte-type fatty acid-binding protein \| AFABP \| ALBP \| ALBP/Ap2 \| aP2 \| Epididymis secretory prot
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity >95% SDS-PAGE Function Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
生物活性	The binding activity of FABP4 with ACTB.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	2-132 aa
序列	CDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA
纯度	＞ 95%(SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	Yes
Accession #	P15090
来源	重组表达
预测分子量	16.2 kDa
SDS-PAGE	16 kDa under reducing condition

图片

Protein Bioactivity: Recombinant Human FABP4 Protein (rp145772)

Recombinant Human FABP4 Protein (rp145772) - Protein Bioactivity
Fatty Acid Binding Protein 4, Adipocyte (FABP4) encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Besides, Actin Beta has been identified as an interactor of FABP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP4 and recombinant human ACTb. The EC₅₀ for this effect is less than 4.23 ug/mL.

SDS-PAGE: Recombinant Human FABP4 Protein (rp145772)

Recombinant Human FABP4 Protein (rp145772) - SDS-PAGE
3μg/lane of Recombinant Human FABP4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.2 kDa.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
复溶	Add 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL.
储存温度	-80℃储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	避免反复冻融循环。在 2-8℃ 下保存一个月。分装后在 -80℃ 下可保存 12 个月。
分子类型	蛋白质

安全和危险性（GHS）

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	日期	货号
ZJ23F0800875	分析证书	23-08-23	rp145772
ZJ23F0800876	分析证书	23-08-23	rp145772
ZJ23F0800877	分析证书	23-08-23	rp145772

参考文献

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2. Liu X, Huang X, Lin W, Wang D, Diao Y, Li H, Hui X, Wang Y, Xu A, Wu D et al.. (2011) New aromatic substituted pyrazoles as selective inhibitors of human adipocyte fatty acid-binding protein.. Bioorg Med Chem Lett, 21 (10): (2949-52). [PMID:21481589]
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4. Haiyan Cai,Guirui Yan,Xiaodong Zhang,Olena Gorbenko,Heyao Wang,Weiliang Zhu. (2010-05-18) Discovery of highly selective inhibitors of human fatty acid binding protein 4 (FABP4) by virtual screening.. Bioorganic & medicinal chemistry letters, 20 ((12)): (3675-3679). [PMID:20471252]
5. Lehmann F, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Lundbäck T, Rondahl L, Barf T.. (2004) Discovery of inhibitors of human adipocyte fatty acid-binding protein, a potential type 2 diabetes target.. Bioorg Med Chem Lett, 14 (17): (4445-4448). [PMID:15357969] [10.1016/j.bmcl.2004.06.057]
6. Barf T, Lehmann F, Hammer K, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Rondahl L, Lundbäck T.. (2009) N-Benzyl-indolo carboxylic acids: Design and synthesis of potent and selective adipocyte fatty-acid binding protein (A-FABP) inhibitors.. Bioorg Med Chem Lett, 19 (6): (1745-1748). [PMID:19217286] [10.1016/j.bmcl.2009.01.084]
7. Cai H, Liu Q, Gao D, Wang T, Chen T, Yan G, Chen K, Xu Y, Wang H, Li Y, Zhu W.. (2015) Novel fatty acid binding protein 4 (FABP4) inhibitors: virtual screening, synthesis and crystal structure determination.. Eur J Med Chem, 90 (241-250). [PMID:25461324] [10.1016/j.ejmech.2014.11.020]
8. Zhou Y, Nie T, Zhang Y, Song M, Li K, Ding M, Ding K, Wu D, Xu Y.. (2016) The discovery of novel and selective fatty acid binding protein 4 inhibitors by virtual screening and biological evaluation.. Bioorg Med Chem, 24 (18): (4310-4317). [PMID:27460668] [10.1016/j.bmc.2016.07.022]
9. Kühne H, Obst-Sander U, Kuhn B, Conte A, Ceccarelli SM, Neidhart W, Rudolph MG, Ottaviani G, Gasser R, So SS, Li S, Zhang X, Gao L, Myers M.. (2016) Design and synthesis of selective, dual fatty acid binding protein 4 and 5 inhibitors.. Bioorg Med Chem Lett, 26 (20): (5092-5097). [PMID:27658368] [10.1016/j.bmcl.2016.08.071]
10. Gao DD, Dou HX, Su HX, Zhang MM, Wang T, Liu QF, Cai HY, Ding HP, Yang Z, Zhu WL, Xu YC, Wang HY, Li YX.. (2018) From hit to lead: Structure-based discovery of naphthalene-1-sulfonamide derivatives as potent and selective inhibitors of fatty acid binding protein 4.. Eur J Med Chem, 154 (44-59). [PMID:29775936] [10.1016/j.ejmech.2018.05.007]
11. Yan S, Elmes MW, Tong S, Hu K, Awwa M, Teng GYH, Jing Y, Freitag M, Gan Q, Clement T, Wei L, Sweeney JM, Joseph OM, Che J, Carbonetti GS, Wang L, Bogdan DM, Falcone J, Smietalo N, Zhou Y, Ralph B, Hsu HC, Li H, Rizzo RC, Deutsch DG, Kaczocha M, Ojima I.. (2018) SAR studies on truxillic acid mono esters as a new class of antinociceptive agents targeting fatty acid binding proteins.. Eur J Med Chem, 154 (233-252). [PMID:29803996] [10.1016/j.ejmech.2018.04.050]
12. He Y, Dou H, Gao D, Wang T, Zhang M, Wang H, Li Y.. (2019) Identification of new dual FABP4/5 inhibitors based on a naphthalene-1-sulfonamide FABP4 inhibitor.. Bioorg Med Chem, 27 (19): (115015-115015). [PMID:31420256] [10.1016/j.bmc.2019.07.031]
13. Oukoloff K, Lucero B, Francisco KR, Brunden KR, Ballatore C.. (2019) 1,2,4-Triazolo[1,5-a]pyrimidines in drug design.. Eur J Med Chem, 165 (332-346). [PMID:30703745] [10.1016/j.ejmech.2019.01.027]
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15. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
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Recombinant Human FABP4 Protein

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基本描述

图片

产品规格参数

安全和危险性（GHS）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

参考文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器