| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp145645-10μg |
10μg |
现货  |
| |
| rp145645-50μg |
50μg |
现货 |
| |
| rp145645-100μg |
100μg |
现货 |
| |
| rp145645-1mg |
1mg |
期货 |
|
| 产品名称 | Recombinant Human Epigen Protein |
|---|---|
| 别名 | 重组人类Epigen蛋白 |
| 英文别名 | EPG | Epithelial mitogen |
| 规格或纯度 | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE&HPLC) |
| 产品介绍 |
Purity >95% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg). Function Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. |
| 生物活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of > 3.3 × 103 IU/mg. |
| 内毒素水平 | <1.0 EU/μg |
| 表达系统 | E. coli |
| 种属 | 人(Human) |
| 氨基酸 | 24-95aa |
| 序列 | AVTVTPPITAQQADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTSYA |
| 纯度 | ＞98% (SDS-PAGE and HPLC) |
| 蛋白标签 | No tag |
| 蛋白长度 | Protein fragment |
| 无载体 | Yes |
| 无动物源 | Yes |
| Accession # | Q6UW88 |
| 来源 | 重组表达 |
| 预测分子量 | 7.9 kDa |
| SDS-PAGE | 7.9 kDa |
Recombinant Human Epigen Protein (rp145645)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/mL, corresponding to a specific activity of > 3.3 × 10³ IU/mg.
Recombinant Human Epigen Protein (rp145645)-SDS-PAGE
Recombinant Human Epigen Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 7.9 kDa.
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4 |
| 复溶 | Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 使用手动解冻冰箱，避免反复冻融循环。自收到之日起 12 个月内，-20 至 -70 °C（按提供时的温度）。1 个月内，复溶后在无菌条件下 2 至 8 °C。3 个月内，复溶后在无菌条件下 -20 至 -70 °C。 |
| 分子类型 | 蛋白质 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ23F0600340 |
分析证书 | 24-03-26 | rp145645 |
| ZJ23F0600338 |
分析证书 | 24-03-26 | rp145645 |
| ZJ23F0600339 |
分析证书 | 24-03-26 | rp145645 |
| 1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10): (2265-70). [PMID:12975309] |
| 2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039] |
| 3. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 4. Kochupurakkal, Bose S BS and 15 more authors.. (2005) Epigen, the last ligand of ErbB receptors, reveals intricate relationships between affinity and mitogenicity.. The Journal of biological chemistry, (4): [PMID:15611079] |
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