| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp142895-10μg |
10μg |
现货  |
| |
| rp142895-50μg |
50μg |
现货 |
| |
| rp142895-100μg |
100μg |
现货 |
| |
| rp142895-1mg |
1mg |
期货 |
|
| 产品名称 | Recombinant Human Apolipoprotein H Protein |
|---|---|
| 别名 | 载脂蛋白H(APOH)重组蛋白 |
| 英文别名 | Activated protein C-binding protein | Anticardiolipin cofactor | APC inhibitor | Apo-H | Apolipoprotein H | B2GPI | Beta(2)GPI | Beta-2-glycoprotein I | Activated protein C-binding protein | Anticardiolipin cofactor | APC inhibitor | APOH | apo-H | apolip |
| 规格或纯度 | Carrier Free, Animal Free, ActiBioPure™, Bioactive, ≥95%(SDS-PAGE) |
| 产品介绍 |
Function |
| 生物活性 | Measured by its binding ability in a functional ELISA. When recombinant human ApoH is immobilized at 2 μg/mL, 100 µL/well, the concentration of recombinant human LDL R that produces 50% of the optimal binding response is found to be approximately 0.2 0.8 μg/mL. |
| 内毒素水平 | <0.1 EU/μg |
| 表达系统 | HEK293 |
| 种属 | 人(Human) |
| 氨基酸 | 20-345 aa |
| 序列 | GRTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPCHHHHHH |
| 蛋白标签 | C-His |
| 无载体 | Yes |
| 无动物源 | Yes |
| Accession # | P02749 |
| 来源 | 重组表达 |
| 预测分子量 | 37.08 kDa |
| SDS-PAGE | 45 - 55 kDa, under reducing conditions; 40 - 50 kDa, under non-reducing conditions. |
Recombinant Human Apolipoprotein H Protein (rp142895) - Protein Bioactivity
Measured by its binding ability in a functional ELISA. When Recombinant Human Apolipoprotein H Protein (rp142895) is immobilized at 2.0 μg/mL, the concentration of recombinant human LDL R that produces 50% of the optimal binding response is found to be approximately 0.63 μg/mL.
Recombinant Human Apolipoprotein H Protein (rp142895) - SDS-PAGE
3 μg/lane of Recombinant Human Apolipoprotein H Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 45 - 55 kDa under reducing condition and 40 - 50 kDa under non-reducing condition.
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4 |
| 复溶 | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 蛋白质 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F1216178 |
分析证书 | 24-12-31 | rp142895 |
| ZJ24F1216177 |
分析证书 | 24-12-31 | rp142895 |
| ZJ24F1216176 |
分析证书 | 24-12-31 | rp142895 |
| 1. Day, J R JR and 6 more authors.. (1992) Molecular cloning and sequence analysis of the cDNA encoding human apolipoprotein H (beta 2-glycoprotein I).. International journal of clinical & laboratory research, [PMID:1339416] |
| 2. Steinkasserer, A A and 6 more authors.. (1992) Activity, disulphide mapping and structural modelling of the fifth domain of human beta 2-glycoprotein I.. FEBS letters, (23): [PMID:1426288] |
| 3. Matsuura, E E and 7 more authors.. (1992) Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:1602135] |
| 4. Steinkasserer, A A, Estaller, C C, Weiss, E H EH, Sim, R B RB and Day, A J AJ.. (1991) Complete nucleotide and deduced amino acid sequence of human beta 2-glycoprotein I.. The Biochemical journal, (15): [PMID:1650181] |
| 5. Kristensen, T T and 7 more authors.. (1991) Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA.. FEBS letters, (9): [PMID:1655523] |
| 6. Mehdi, H H and 6 more authors.. (1991) Nucleotide sequence and expression of the human gene encoding apolipoprotein H (beta 2-glycoprotein I).. Gene, (15): [PMID:1748314] |
| 7. Matsuura, E E and 6 more authors.. (1991) Molecular definition of human beta 2-glycoprotein I (beta 2-GPI) by cDNA cloning and inter-species differences of beta 2-GPI in alternation of anticardiolipin binding.. International immunology, [PMID:1777418] |
| 8. McNeil, H P HP, Simpson, R J RJ, Chesterman, C N CN and Krilis, S A SA.. (1990) Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H).. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2349221] |
| 9. Lozier, J J, Takahashi, N N and Putnam, F W FW.. (1984) Complete amino acid sequence of human plasma beta 2-glycoprotein I.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6587378] |
| 10. Steinkasserer, A A, Dörner, C C, Würzner, R R and Sim, R B RB.. (1993) Human beta 2-glycoprotein I: molecular analysis of DNA and amino acid polymorphism.. Human genetics, [PMID:8099061] |
| 11. Sanghera, D K DK, Wagenknecht, D R DR, McIntyre, J A JA and Kamboh, M I MI.. (1997) Identification of structural mutations in the fifth domain of apolipoprotein H (beta 2-glycoprotein I) which affect phospholipid binding.. Human molecular genetics, [PMID:9063752] |
| 12. Gambino, R R, Ruiu, G G, Pagano, G G and Cassader, M M.. (1997) Qualitative analysis of the carbohydrate composition of apolipoprotein H.. Journal of protein chemistry, [PMID:9155091] |
| 13. Sanghera, D K DK, Kristensen, T T, Hamman, R F RF and Kamboh, M I MI.. (1997) Molecular basis of the apolipoprotein H (beta 2-glycoprotein I) protein polymorphism.. Human genetics, [PMID:9225969] |
| 14. Okkels, H H, Rasmussen, T E TE, Sanghera, D K DK, Kamboh, M I MI and Kristensen, T T.. (1999) Structure of the human beta2-glycoprotein I (apolipoprotein H) gene.. European journal of biochemistry, [PMID:9914524] |
| 15. Bouma, B B and 8 more authors.. (1999) Adhesion mechanism of human beta(2)-glycoprotein I to phospholipids based on its crystal structure.. The EMBO journal, (1): [PMID:10508150] |
| 16. Schwarzenbacher, R R and 6 more authors.. (1999) Crystal structure of human beta2-glycoprotein I: implications for phospholipid binding and the antiphospholipid syndrome.. The EMBO journal, (15): [PMID:10562535] |
| 17. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718] |
| 18. Kristiansen, Troels Zakarias TZ and 8 more authors.. (2004) A proteomic analysis of human bile.. Molecular & cellular proteomics : MCP, [PMID:15084671] |
| 19. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 20. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002] |
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