重组HIV逆转录酶

EnzymoPure™

≥5,000 units/mg protein

功能和特点

表达系统: E. coli
Accession #: P04585

有货

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货号 (SKU)	包装规格	是否现货	价格	数量
R128560-200UN	200U	现货

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Accession#:P04585 (1) gag-pol (1) Gene ID:155348 (1) Reverse Transcriptase (71) 酶类 (34)

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基本描述

产品名称	重组HIV逆转录酶
英文别名	HIV 1 Reverse transcriptase \| HIV1 RT \| Human Immunodeficiency virus 1 \| p51 RT \| p66 RT \| Reverse transcriptase p51 subunit \| Reverse transcriptase p66 subunit \| gag-pol \| ab63979
规格或纯度	EnzymoPure™, ≥5,000 units/mg protein
产品介绍	Reverse transcriptases are enzymes encoded in retroviruses viral genome. The enzyme is responsible for transcription of the viral RNA to produce a dsDNA that can be inserted into the host genome. Reverse transcriptases are multifunctional enzymes. These enzymes exhibit an RNA and DNA directed polymerase activity. In addition reverse transcriptases catalyze the degradation of RNA in an RNA-DNA hybrid. The exonucleolytic activity proceeds in a 5' ---> 3' direction. The RNA or DNA directed activity requires a template (RNA or DNA) and a primer. The following is a schematic illustration of the reaction: Unit definition: One unit incorporates 1 nanomole of tritiated dTMP into acid insoluble productsusing poly(A)•oligo(dT) 12-18 as the template-primer in 20 minutes at 37° C. Applications HIV reverse transcriptase is used for research on the AIDS primer. However it can be substituted for AMV reverse transcriptase, which is mainly used to transcribe mRNA into double stranded cDNA, that can be inserted into prokaryotic vectors. The enzyme can also be used with either single stranded DNA or RNA templates to make probes for use in hybridization experiments. It can be used for labeling the termini of DNA fragments with protruding 5' termini. The enzyme can also be used to sequence DNAs by the dideoxy chain termination method of Sanger when the Klenow fragment of E. coli DNA polymerase I, or the T7 DNA polymerase yield unsatisfactory results. Reagents 0.05 M Tris, pH 8.3, containing 0.008 M MgCl2 1 mg/ml polyadenylic acid in water (poly A) DNA primer:Oligo d(T)12-18 1 μ mole dTTP/mL stock solution [methyl-3H]-Thymidine 5'-triphosphate (3H-dTTP) dTTP-3H-dTTP working mix: Add 1-2 μL 3H-dTTP per mL of 100 nmol/mL dTTP in order to obtain 1 to 1.5 x 105 cpm/mL 1% bovine serum albumin 10% perchloric acid 1% perchloric acid Buffer substrate reaction mixture: Prepare fresh, immediately before use: For each 1mL of reaction mixture required mix: 0.7 mL Tris/HCl, pH 8.3, 0.008M MgCl2 0.3 mL 1 mg/mL poly(A) RNA template 0.005 mL 0.02 mg/mL oligo d(T)12-18 DNA primer 0.02mL 1% BSA Enzyme dilute as needed wtih 0.05M Tris/HCl, pH 8.3, 0.008M MgCl2 containing 0.1 mg/mL (1%) BSA Procedure Pipette into each tube as follows: Buffer substrate mix：0.1 mL dTTP-3H3-dTTP：0.1 mL Enzyme：5-10 μL Incubate 20 minutes at 37° C. Stop reaction by adding 1 ml 10% cold perchloric acid. Filter through 0.2μ manifold filters used with Millipore vacuum manifold. Wash four times using 2mL 1% cold perchloric acid/wash. Transfer filter to scintillation vials. Add 2mL Cellosolve (or 2-methoxyethanol) to dissolve filter. Filters become opaque upon addition of Cellosolve. Make sure filters are dissolved before proceeding. Add 10mL scintillation cocktail and count. Calculation
表达系统	E. coli
种属	HIV
Accession #	P04585
来源	重组表达

产品规格参数

物理形态	液体
储存缓冲液	10mM Potassium phosphate, 1mM DTT, 20% Glycerol, pH 7.4.
浓度	≥5,000 units/mg protein
储存温度	-20°C储存
运输条件	超低温冰袋运输
CAS编号和信息	9068-38-6
酶学委员会编号	2.7.7.49
单位定义	One Unit incorporates 1 nmole of tritiated d-TMP into acid precipitable products using poly(A)/oligo(dT)12-18 as the template/primer in 20 minutes at 37°C, pH 8.3.
分子类型	酶

安全和危险性（GHS）

象形图	GHS08, GHS07
信号词	Warning
预防措施声明	P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P342+P311: 如有呼吸系统病症：呼叫急救中心/医生。
WGK Germany	3
个人防护装备	Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR	NONH for all modes of transport

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批号(Lot Number)	证书类型	日期	货号
C2426339	分析证书	24-03-14	R128560
I1818102	分析证书	22-07-21	R128560
I1818101	分析证书	22-07-21	R128560

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