核酸酶S1 来源于米曲霉

功能和特点
有货

库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
N128634-10KU
10KU 现货 Stock Image
N128634-50KU
50KU 现货 Stock Image

基本描述

产品名称 核酸酶S1 来源于米曲霉
别名 Endonuclease S1
英文别名 Endonuclease S1
规格或纯度 EnzymoPure™, ≥100,000-500,000 units/ml
生化机理 从黑曲霉(Aspergillus oryzae)中分离出的核酸酶 S1 对单链 DNA 和 RNA 的磷酸二酯键具有内切和外切水解活性,可产生 5'-phosphomononucleotide 和 5'-phosphooligonucleotide 终产物。它可用于消化 RNA 和 DNA 双链中未退火的多核苷酸尾部和发夹环,也可用于将超螺旋 DNA 转换为线性形式。
产品介绍


Product Description
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.

Product Application
Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

产品规格参数

浓度 ≥100,000-500,000 units/ml
储存温度 -20°C储存
运输条件 超低温冰袋运输
CAS编号和信息 37288-25-8
酶学委员会编号 3.1.30.1
单位定义 One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.

安全和危险性(GHS)

WGK Germany 2

质量标准

Enzyme activity ≥100000(IU/ml OR u/ml)

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找到5个结果

批号(Lot Number) 证书类型 日期 货号
D23261285 分析证书 24-02-22 N128634
D23261286 分析证书 24-02-22 N128634
G2229367 分析证书 23-05-10 N128634
G2229368 分析证书 23-05-10 N128634
I1507106 分析证书 23-04-14 N128634

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