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切割、合成、连接和修饰
选项
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Poly(U)聚合酶
- 种属: 粟酒裂殖酵母(Schizosaccharomyces pombe)
- Accession #: O13833.2
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme that incorporates 1nmol of UMP into RNA in a 50μl volume in 10 minutes at 37ºC.
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T4 RNA 连接酶 2，截短型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.
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T4 RNA 连接酶 2，截短突变型
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P32277.1
- 表达系统: E. coli
- 生物活性: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
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UTP (100mM, Tris Buffered)分子式: C9H15N2O15P3(free acid) 分子量: 484.14 (free acid)
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ATP (100mM, Tris Buffered)分子式: C10H16N5O13P3(free acid) 分子量: 507.18 (free acid)
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CTP (100mM, Tris Buffered)分子式: C9H16N3O14P3(free acid) 分子量: 483.16 (free acid)
