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克隆和蛋白表达
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超快速T4 DNA连接酶
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P00970
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA in 30min at 16℃ in 20µl of the assay mixture containing 50mM Tris, pH7.5, 10mM MgCl2, 10mM DTT, 1mM ATP, 25µg/ml BSA and a 5'-DNA termini concentration of 0.12µM (300µg/ml).200U equals 1 Weiss unit， Based on Weiss unit, the packaging of this product is 200U and 1000U respectively.
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T4 多聚核苷酸激酶 (3'磷酸酶活性缺失)
- 种属: Escherichia phage T4
- Accession #: NP_049834.1
- 表达系统: E. coli
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末端脱氧核糖核酸转移酶 (TdT, 20U/μL)
- 种属: 人(Human)
- Accession #: P04053
- 表达系统: E. coli
- 生物活性: One unit of enzyme activity (U) is defined as the amount of enzyme required to catalyze the addition of 1 nmol of dNTP to the 3'-hydroxyl end of a polynucleotide in 60 minutes at 37°C.
