甘油3-磷酸氧化酶

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货号 (SKU) 包装规格 是否现货 价格 数量
G196970-0.5KU
0.5KU 现货 Stock Image
G196970-1KU
1KU 现货 Stock Image
G196970-5KU
5KU 现货 Stock Image
G196970-10KU
10KU 现货 Stock Image
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磷酸氧化酶 (1) 酶类 (34)

基本描述

产品名称 甘油3-磷酸氧化酶
别名 甘油3-磷酸氧化酶 | sn-3-磷酸甘油:2-氧氧化还原酶 | 3-磷酸​​甘油氧化 | 酶
英文别名 sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase | L-Glycerol 3-phosphate Oxidase
规格或纯度 EnzymoPure™, ≥35U/mg
产品介绍

活力定义:

One unit is defined as the enzyme quantity which oxidizes one μ mole of L-a-Glycerophosphate per minute under the condition described below.
Reagents
A. Aminoantipyrine Solution (5 mg/mi)
Weigh 0.25 g of s-aminoantipyrinc and dissoive in 20 ml ol dcionized water. Fill up to 50ml with deionized water.
B. Phenol Solution (50 mg/ ml)
Weigh 5.0 g of phenol and dissolve in 30 ml of deionized water. Fill up to 100 ml with deionized water.
C. 0.2M Phosphate Buffer (KH2PO4-NaOH, pH 20)
D. 002M Phosphate Buffer (KH2PO4-NaOH, pH 20)
E. Aminoantipyrine-Phenol Solution
Dilute 6 ml of Aminoantipyrine Solution (A), 0.5 ml of Phenol Solution (B) and 200 purpurogallin units of Per-oxidase (produced by Amano Enzyme Inc.) with 0.2M Phosphate Buffer (C). Fill up to 50 ml with 0.2M Phosphate Buffer (C). (Expires after 14 days at 2-8°C)
F. Substrate Solution
Weigh 36.7 g of DL-α-glycerophosphate·2Na·5H2O (produced by Wako Pure Chemical Industries, Ltd. *) and dissolve in approx. 40 ml of deionized water. Adjust the pH to 7.0 with 4N HCl and fill up to 100 ml with deionized water. Then, filtrate the solution with 0.45 um membrane filter.
G. Diluent
Weigh 1.00 g of bovine serum albumin (produced by Wako Pure Chemical Industries, Ltd.: Fraction V*), 66.0 g of (NH,),SO, and dissolve in 002M Phosphate Buffer (D). Fil up to 1,000 ml with 0.02M Phosphate Buffer (D). (Expires after 1 month at 2-8°C)
H. Enzyme Solution
Weigh 20 mg of L-a-Glycerophosphate Oxidase "Amano" and dissolve in chilled Diluent (G).
Enzyme Solution should be prepared so that the value of △OD/ minute becomes in the range of 0.020±0.005


甘油三酯的氧化 用于医学检测甘油三酯

This enzyme is used for the enzymatic determination of triglyceride in serum by coupling with lipoprotein lipase and glycerol kinase in clinical diagnosis.

产品规格参数

浓度 ≥35U/mg
储存温度 -20°C储存
运输条件 超低温冰袋运输
CAS编号和信息 9046-28-0
酶学委员会编号 EC 1.1.3.21

安全和危险性(GHS)

WGK Germany 3
个人防护装备 Eyeshields, Gloves, type N95 (US)
RIDADR NONH for all modes of transport

质量标准

Appearance(G196970) Yellow powder, lyophilized
Activity :GPO-3 ≥35(U/mg)
Lactate oxidase 0-0.003(%)
Catalase 0-0.004(%)
ATPase 0-0.006(%)
Phosphatase 0-0.0004(%)

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到9个结果

批号(Lot Number) 证书类型 日期 货号
F2413115 分析证书 25-04-18 G196970
F2413117 分析证书 25-04-18 G196970
F2413121 分析证书 25-04-18 G196970
F2413119 分析证书 25-04-18 G196970
F2413116 分析证书 25-03-04 G196970
F2413118 分析证书 25-03-04 G196970
F2413120 分析证书 25-03-04 G196970
F2413122 分析证书 25-03-04 G196970
F2412119 分析证书 24-05-21 G196970

此产品的引用文献

1. Jia Xu, Habib Khan, Li Yang.  (2021)  Hydrogel Paper-Based Analytical Devices: Separation-Free In Situ Assay of Small-Molecule Targets in Whole Blood.  ANALYTICAL CHEMISTRY,  93  (44): (14755–14763).  [PMID:34709797] [10.1021/acs.analchem.1c03347]

参考文献

1. Jia Xu, Habib Khan, Li Yang.  (2021)  Hydrogel Paper-Based Analytical Devices: Separation-Free In Situ Assay of Small-Molecule Targets in Whole Blood.  ANALYTICAL CHEMISTRY,  93  (44): (14755–14763).  [PMID:34709797] [10.1021/acs.analchem.1c03347]

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