基本描述

规格或纯度

2N

稳定性与储存

4℃避光保存，一年有效。室温保存，至少一个月内有效。

英文名称

Folin & Ciocalteu's Phenol Reagent

储存温度

2-8°C储存,避光

运输条件

冰袋运输

产品介绍

本试剂采用钨酸钠、钼酸钠、磷酸、浓盐酸按一定比例加热回流后加入硫酸锂精制而成，色泽金黄、透亮，是实验室测定溶液中蛋白质、多肽、多酚或酪氨酸等物质含量的常用试剂。本品酸度为2N，请根据实验需要直接使用或适当稀释。本试剂可测定蛋白浓度 0.05～1mg/mL，当蛋白浓度范围为0.05～0.5mg/mL 时，线性关系最好。如果待测样品蛋白浓度超过此范围，请适当稀释到合适浓度范围。本试剂具有腐蚀性，应避免接触皮肤、粘膜等；试剂颜色变为墨绿色时应弃用。本试剂为 2N 浓度，使用时可根据具体要求用蒸馏水或者去离子水进行稀释。该产品仅适用于科研领域，禁止用于临床诊断或其他用途。注意密封保存，避免挥发和腐蚀物品。

实验前准备工作：
1、配制 A1：称取 20g 无水碳酸钠、4g 氢氧化钠溶解于 1L 水中；
2、配制 A2：称取 0.2g 硫酸铜溶于 20mL 水中；
3、配制 A3：称取 0.4g 酒石酸钾钠溶于 20mL 水中；在测定的当天将A1:A2:A3 按100:1:1的体积比混合，即为试剂 A，混合放置 30 分钟后使用。先将 A2 和 A3 混合，混合液再与A1 混合，次序颠倒可能有沉淀产生。试剂 A 只能当天使用，如产生沉淀或混浊需重新配制，三种溶液分开于室温下可长期保存。
4、先制作 2mg/ml 的蛋白母液，再参考“附表”稀释成各种浓度的蛋白标准品溶液。5、福林酚试剂 2N 稀释至 1N 使用。

操作步骤操作步骤（反应总体积 5.6ml）：

/	样品编号标	标0	标1	标2	标3	标4	标5	标6	待测
1.加样	加入蛋白标准品或待测蛋白样品(mL)	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
/	蛋白浓度(mg/mL)	0	0.05	0.10	0.20	0.25	0.40	0.50	待测
2.加A	各管依次加入试剂 A5ml;混匀后室温放置 10 分钟;	/	/	/	/	/	/	/	/
3.加B	各管依次加入试剂 B(福林酚试剂)0.5m(每加一管必须立即混匀);室温放置 30 分钟;	/	/	/	/	/	/	/	/
4.测定	750nm 波长光下，以标0号管对设备进行调零，测定每一管的光吸收度(OD 值);	/	/	/	/	/	/	/	/
5.计算	根据标 0~标6号管所测数据绘制标准曲线，计算出各待测样品浓度(如果待测样品浓度超出标准曲线范围，请适当稀释后重新检测)。	/	/	/	/	/	/	/	/

注：根据实际所需反应总体积，以上试剂用量可按比例缩放（蛋白样品：试剂 A：福林酚试剂＝1：50：5）

This reagent is made by heating and refluxing sodium tungstate, sodium molybdate, phosphoric acid, and concentrated hydrochloric acid in a certain proportion, and then refining them with lithium sulfate. It has a golden and transparent color and is a commonly used reagent for determining the content of proteins, peptides, polyphenols, tyrosine, and other substances in laboratory solutions. The acidity of this product is 2N. Please use it directly or dilute it appropriately according to experimental needs. This reagent can determine protein concentrations ranging from 0.05 to 1mg/mL. The linear relationship is best when the protein concentration is within the range of 0.05 to 0.5mg/mL. If the protein concentration of the test sample exceeds this range, please dilute it appropriately to the appropriate concentration range. This reagent is corrosive and should be avoided from contact with skin, mucous membranes, etc; When the color of the reagent turns dark green, it should be discarded. This reagent has a concentration of 2N and can be diluted with distilled water or deionized water according to specific requirements during use. This product is only suitable for scientific research and is prohibited from being used for clinical diagnosis or other purposes. Pay attention to sealed storage to avoid volatile and corrosive substances.
Preparation before the experiment:
1. Preparation A1: Weigh 20g anhydrous sodium carbonate and 4g sodium hydroxide and dissolve them in 1L of water;
2. Preparation A2: Weigh 0.2g of copper sulfate and dissolve it in 20mL of water;
3. Preparation A3: Weigh 0.4g of potassium sodium tartrate and dissolve it in 20mL of water; On the day of measurement, mix A1: A2: A3 in a volume ratio of 100:1:1 to obtain reagent A. Mix and let it sit for 30 minutes before use. Mix A2 and A3 first, and then mix the mixture with A1. Reversing the order may result in precipitation. Reagent A can only be used on the same day. If precipitation or turbidity occurs, it needs to be re prepared. The three solutions can be stored separately at room temperature for a long time.
4. First, prepare a 2mg/ml protein mother liquor, and then dilute it into protein standard solutions of various concentrations according to the "Appendix". 5. Dilute the Folin phenol reagent from 2N to 1N for use.
Operation steps (total reaction volume 5.6ml):

/	Sample number label	Label 0	Label 1	Label 2	Label 3	Label 4	Label 5	Label 6	To be tested
1.Add sample	Add protein standard or test protein sample (mL)	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
/	Protein concentration (mg/mL)	0	0.05	0.10	0.20	0.25	0.40	0.50	To be tested
2.Add A	Add A5ml of reagent to each tube in sequence; After mixing, let it stand at room temperature for 10 minutes;	/	/	/	/	/	/	/	/
3.Add B	Add 0.5m of reagent B (Folin phenol reagent) to each tube in sequence (mix immediately for each tube added); Leave at room temperature for 30 minutes;	/	/	/	/	/	/	/	/
4.Measurement	Under 750nm wavelength light, zero the equipment with a standard tube and measure the absorbance (OD value) of each tube;	/	/	/	/	/	/	/	/
5.Calculation	Draw a standard curve based on the data measured by tubes 0 to 6, and calculate the concentration of each sample to be tested (if the concentration of the sample exceeds Standard curve range, please dilute appropriately and retest.	/	/	/	/	/	/	/	/

Note: The above reagent quantities can be scaled proportionally based on the actual required total reaction volume (protein sample: reagent A: Folin phenol reagent=1:50:5)

名称和识别符

分子类型	未知

化学和物理性质

敏感性	对光线敏感

安全和危险性（GHS）

SDS英文版

质量标准

Determination of protein quality	Pass
Concentraion	1.9-2.1（mol/L）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到11个结果

批号(Lot Number)	证书类型	日期	货号
E2520470	分析证书	25-05-12	F743373
E2520477	分析证书	25-05-12	F743373
B2527411	分析证书	25-02-12	F743373
B2512466	分析证书	25-01-09	F743373
B2512467	分析证书	25-01-09	F743373
K2429322	分析证书	24-11-22	F743373
K2429336	分析证书	24-11-22	F743373
K2429337	分析证书	24-11-22	F743373
K2429338	分析证书	24-11-22	F743373
J2431355	分析证书	24-10-26	F743373
J2431356	分析证书	24-10-26	F743373

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货号 (SKU)	包装规格	是否现货	价格	数量
F743373-100ml	100ml	现货
F743373-500ml	500ml	现货

福林酚试剂

库存信息

库存信息

基本描述

名称和识别符

化学和物理性质

安全和危险性（GHS）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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