| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| F666101-500U |
500U |
期货  |
| |
| F666101-5000U |
5000U |
期货 |
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| 别名 | FastStar 探针试剂盒（用于双 DNA） | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 英文名称 | FastStar Probe Kit (for bisDNA) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 储存温度 | 避光,-20°C储存,避免反复冻融 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 超低温冰袋运输 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
产品内容
产品简介
本产品主要应用于以亚硫酸氢盐处理的DNA为模板的探针法荧光定量PCR。其中 SuperFastStar DNA Polymerase是一种经双单克隆抗体修饰的、全新高效热启动酶，在 常温下酶的活性被完全封闭，从而有效避免在常温条件下由引物和模板非特异性结合或 引物二聚体而产生的非特异性扩增，搭配优化的FastStar Probe Buffer (for bisDNA)包含 PCR Buffer、dNTPs 和Mg2+等，只需客户加入模板，引物，探针即可，使用方便。 4．需自备乙醇，冰醋酸。 注意事项 1.使用前请在本品完全融化后上下颠倒轻轻混匀，并经短暂离心后使用。 2.避免反复冻融本品，反复冻融可能使产品性能下降。本品可置于-20℃长期保存，
避光。如果在短期内需要频繁使用，可在2-8℃保存。
使用方法 以下举例为常规PCR反应体系和反应条件，实际操作中应根据模板、引物结构和 目的片段大小不同进行相应的改进和优化。 1. PCR反应体系
注意： 1）通常引物浓度以0.2 μM可以得到较好结果，可以在0.1-1.0 μM作为设定范围的参考。 2）使用的探针浓度，与使用的荧光定量PCR仪、探针种类、荧光标记物质种类有关，实际使用时请参照 仪器说明书，或各荧光探针的具体使用要求进行浓度的调节。 3）通常DNA模板的量以10-100 ng基因组DNA或1-10 ng cDNA为参照，因不同物种的模板中含有的目 的基因拷贝数不同，可对模板进行梯度稀释，以确定最佳的模板使用量。 2. PCR反应条件
注意：1）本产品95℃初始变性30s足以使酶激活；复杂模板可延长至3min变性。
2）建议采用两步法PCR反应程序，若因使用Tm值较低的引物等原因，得不到良好的实验结果时，可尝
试进行三步法PCR扩增，退火温度请以 56℃-64℃的范围作为设定参考。
Products Content:
Products Introduction This product is mainly used for PCR using bisulfite-treated DNA as template, in which SuperFastStar DNA Polymerase is a new high-efficiency hot-start enzyme modified by bis-monoclonal antibody, which is completely blocked at room temperature, thus effectively avoiding non-specific amplification caused by the non-specific binding of the primer to the template or the primer dimerization under the condition of room temperature. The optimized FastStar Probe Buffer (for bisDNA) contains PCR Buffer, dNTPs and Mg2+, etc., which is easy to use as customers only need to add templates, primers and probes. caveat 1 Before use, please mix the product gently by turning it up and down after it has been completely melted and centrifuged briefly. 2. Avoid repeated freezing and thawing of the product, which may degrade its performance. This product can be stored at -20℃ for a long period of time, protected from light. If frequent use is required within a short period of time, it can be stored at 2-8℃. Usage The following examples are conventional PCR reaction systems and conditions, which should be improved and optimized according to the template, primer structure and target fragment size. 1.PCR reaction system Note: 1) Usually, better results can be obtained with a primer concentration of 0.2 μM, and 0.1-1.0 μM can be used as a reference for setting the range. 2）The concentration of the probe used is related to the fluorescence quantitative PCR instrument used, the type of probe, and the type of fluorescent labeling substance, so please refer to the instrument manual or the specific requirements for the use of each fluorescent probe to adjust the concentration. 3）Usually the amount of DNA template is 10-100 ng of genomic DNA or 1-10 ng of cDNA as a reference. Since the templates of different species contain different copy numbers of the target gene, the templates can be diluted in gradients to determine the optimal amount of template to use. 2.PCR reaction conditions
Note: 1) The initial denaturation of this product at 95°C for 30s is sufficient for enzyme activation; complex templates can be extended to 3min denaturation. (2) It is recommended to use two-step PCR reaction program, if you can't get good experimental results due to the use of primers with lower Tm value, etc., you can try three-step PCR amplification, and the annealing temperature should be set in the range of 56℃-64℃ as a reference.
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