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Earle's Balanced Salt Solution (with Ca2+ & Mg2+, 自噬诱导试剂)
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库存信息
| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| E743125-500ml |
500ml |
期货  |
|
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基本描述
| 稳定性与储存 | 4℃保存，半年有效。-20℃保存一年有效。本产品可以短期室温存放。 |
|---|---|
| 英文名称 | Earle's Balanced Salt Solution (with Ca2+xa0& Mg2+) |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
阿拉丁生产的Earle's Balanced Salt Solution (with Ca"2+& Mg2+, 自噬诱导试剂)，简称EBSS with Ca2+& Mg2+，含117.24mM NaCl, 5.33mM KCl, 26.19mM NaHCO3, 1.01mM NaH2PO4, 1.80mM CaCl2, 0.813mM MgSO4, 5.56mM D-Glucose。本Earle's Balanced Salt Solution溶液经过过滤除菌，可以直接用于细胞培养过程中细胞的洗涤、溶液的配制、细胞计数时的稀释等常规用途。使用前不必再进行稀释或过滤除菌等任何处理。本产品简称EBSS，常用于诱导细胞自噬。诱导细胞自噬时，吸净细胞培养液，用EBSS洗涤1-3次，然后在EBSS中孵育例如10、20、40或60min就可以诱导细胞发生非常显著的自噬。多次充分洗涤细胞，可以确保发生非常显著的自噬。不同细胞诱导发生自噬所需的时间有所不同，初次实验需要适当摸索一下诱导自噬的时间。推荐使用阿拉丁生产的C3012 AdPlus-mCherry-GFP-LC3B、C3011 Ad-mCherry-GFP-LC3B、AF5225 LC3B Rabbit Polyclonal Antibody (KO Validated)或AL221 LC3B抗体(兔多抗)检测细胞自噬。4℃保存，半年有效。 Earle's Balanced Salt Solution (with Ca2+ & Mg2+), abbreviated as EBSS (with Ca2+ & Mg2+), produced by aladdin contains 117.24mM NaCl, 5.33mM KCl, 26.19mM NaHCO3, 1.01mM NaH2PO4, 1.80mM CaCl2, 0.813mM MgSO4, and 5.56mM D-Glucose. It is sterile and can be directly used for autophage induction, cell washing, solution preparation, and cell counting dilutions etc. Precautions： Use aseptic techniques while handling this product to avoid microorganism contamination.This product contains Ca2+ and Mg2+. also provides EBSS without Ca2+ and Mg2+ .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear lab coat and disposable gloves during the operation.Autophage Induction with EBSS (with Ca2+ & Mg2+)：1. Grow cells in appropriate vessels.2. To induce cell autophagy, remove the culture medium and wash cells 1-3 times with EBSS (with Ca2+ & Mg2+).3. Add appropriate amount of EBSS (with Ca2+ & Mg2+) and incubate for 10, 20, 40 or 60 minutes to induce autophagy. Note: The duration of autophagy induction varies, which needs to be optimized for different cells.4. Wash cells thoroughly to ensure a significant autophagy. It is recommended to detect autophage with AdPlus-mCherry-GFP-LC3B , Ad-mCherry-GFP-LC3B , LC3B Rabbit Polyclonal Antibody (KO Validated) or LC3B antibody (KO Validated) produced by . |
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