基本描述

产品名称

Bovine Thrombin

别名

牛凝血酶

规格或纯度

高级纯, > 2200 U/Mg Protein

产品介绍

Test	Specification
Thrombin Activity (by coagulation)	> 200,000 US units/g powder
Protein (by Biuret)	not specified
Thrombin Specific Activity	> 2200 US units/mg powder
Moisture	0 - 5%
pH (1% solution)	6.5 - 7.5
Salt Environment	Glycine, Sodium Chloride
Recommended diluent for reconstitution	0.9% NaCl

This product is forin vitro (diagnostic) use only. Not for human or animal consumption.

The high purity bovine thrombin (> 2200 U/mg protein) is used by many diagnostic companies worldwide to defibrinate plasma, producing serum matrixes to be used as controls and standards or in polyclonal antibody production. Using bovine thrombin in polyclonal antibody production to defibrinate plasma may increase antibody yields by 15-20% over off-the-clot methods of defibrination.

Our high purity thrombin is also used to cleave His Tags and GST Tags with a high degree of specificity. We express our units in US Units (aka, NIH Units) measured by coagulation. There are approximately 5 Thrombin cleavage units for every one US Unit.

产品属性

Nacres	NA.54

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

产品规格参数

浓度	> 2200 U/Mg Protein
CAS编号和信息	9002-04-4
酶学委员会编号	3.4.21.5 (BRENDA, IUBMB)
分子类型	小分子

安全和危险性（GHS）

象形图	GHS08, GHS07
信号词	Danger
危险声明	H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H335: 可能引起呼吸道刺激 H334: 吸入可能引起过敏或哮喘病症状或呼吸困难
预防措施声明	P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P405: 密闭存放 P501: 将内容物/容器处理到。。。 P264: 处理后要彻底洗手。 P284: 如果通风不良，请佩戴呼吸防护装置。 P271: 仅在室外或通风良好的地方使用。 P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。 P403+P233: 存放在通风良好的地方。保持容器密闭。 P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。 P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。 P337+P317: 如果眼睛刺激持续：寻求医疗帮助。 P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。 P319: 如果你感到不适，请寻求医疗帮助。 P342+P316: 如果出现呼吸道症状：立即寻求急救。

SDS英文版

质量标准

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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此产品的引用文献

如何在您的发表作品中引用阿拉丁的产品和网站?

1. Zhechang Chen, Caijie Yuan, Yaxin Ye, Bitao Lu, Enling Hu, Fei Lu, Kun Yu, Ruiqi Xie, Guangqian Lan. (2024) Dual-targeting fucoidan-based microvesicle for arterial thrombolysis and re-occlusion inhibition. CARBOHYDRATE POLYMERS, 328 (121703). [PMID:38220339] [10.1016/j.carbpol.2023.121703]
2. Hong Liu, Xuliang Yang, Bo Huang, Hongxiang Liu. (2023) A universal approach for synthesis of copper nanoclusters templated by G-rich oligonucleotide sequences and their applications in sensing. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 297 (122740). [PMID:37080047] [10.1016/j.saa.2023.122740]
3. Xinxin Ren, Qingqing Zhang, Lingxia Qin, Kaiyue Hu, Zhiyong Guo, Sui Wang, Yufang Hu. (2023) Checkerboard arranged G4 nanostructure-supported electrochemical platform and its application to unique bio-enzymes examination. BIOELECTROCHEMISTRY, 149 (108282). [PMID:36242949] [10.1016/j.bioelechem.2022.108282]
4. Xinxin Ren, Kaiyue Hu, Lingxia Qin, Di Wu, Zhiyong Guo, Sui Wang, Yufang Hu. (2022) Development of ZnO nanoflowers-assisted DNAzyme-based electrochemical platform for invertase and glucose oxidase-dominated biosensing. ANALYTICA CHIMICA ACTA, 1232 (340438). [PMID:36257731] [10.1016/j.aca.2022.340438]
5. Songzhi Xie, Chuanfei Mo, Wenxiong Cao, Shuang Xie, Shang Li, Zhanlin Zhang, Xiaohong Li. (2022) Bacteria-propelled microtubular motors for efficient penetration and targeting delivery of thrombolytic agents. Acta Biomaterialia, 142 (49). [PMID:35158079] [10.1016/j.actbio.2022.02.008]
6. Fuad Abduro Bushira, Shimeles Addisu Kitte, Yong Wang, Haijuan Li, Ping Wang, Yongdong Jin. (2021) Plasmon-Boosted Cu-Doped TiO2 Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase. ANALYTICAL CHEMISTRY, 93 (45): (15183–15191). [PMID:34743510] [10.1021/acs.analchem.1c03842]
7. Qing Zhu, Ping Ye, Fang Guo, Yimen Zhu, Wenbin Nan, Zhaohua Chang. (2021) A heparin-functionalized covered stent prepared by plasma technology. JOURNAL OF BIOMATERIALS APPLICATIONS, [PMID:34672223] [10.1177/08853282211051871]
8. Liu Shanshan, Zheng Sitian, Chu Jing. (2021) Cationic Polythiophene-based Colorimetric Assay for Probing the Activity of Protein Kinase A. ANALYTICAL SCIENCES, 37 (7): (1039-1043). [PMID:33250451] [10.2116/analsci.20N034]
9. Zheng Di-Wei, Hong Sheng, Zhang Qiu-Ling, Dong Xue, Pan Pei, Song Wen-Fang, Song Wen, Cheng Si-Xue, Zhang Xian-Zheng. (2020) Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies. Nature Communications, 11 (1): (1-15). [PMID:32999289] [10.1038/s41467-020-18493-7]
10. Zhang Hong, Liu Pengfei, Wang Huifang, Ji Xiaoming, Zhao Mingqin, Song Zhaopeng. (2021) Label-free fluorescent sensor for one-step lysozyme detection via positively charged gold nanorods. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 413 (6): (1541-1547). [PMID:32705288] [10.1007/s00216-020-02814-2]
11. Liping Lin, Yaxin Luo, Qiushuang Chen, Qingjiao Lai, Qiaoling Zheng. (2020) Redox-modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles. LUMINESCENCE, 35 (4): (542-549). [PMID:31898408] [10.1002/bio.3749]
12. Ding Wang, Yaqin Chai, Yali Yuan, Ruo Yuan. (2020) Lattice-Like DNA Tetrahedron Nanostructure as Scaffold to Locate GOx and HRP Enzymes for Highly Efficient Enzyme Cascade Reaction. ACS Applied Materials & Interfaces, 12 (2): (2871–2877). [PMID:31849211] [10.1021/acsami.9b18702]
13. Wang Jing, Li Bingzhi, Lu Qiaoyun, Li Xiaoyun, Weng Chenyuan, Yan Xiaoqiang, Hong Junli, Zhou Xuemin. (2019) A versatile fluorometric aptasensing scheme based on the use of a hybrid material composed of polypyrrole nanoparticles and DNA-silver nanoclusters: application to the determination of adenosine, thrombin, or interferon-gamma. MICROCHIMICA ACTA, 186 (6): (1-8). [PMID:31098714] [10.1007/s00604-019-3459-9]
14. Fangjing Mo, Jingling Wu, Min Chen, Hui Meng, Qian Han, Yingzi Fu. (2019) Enzyme-free “on-off-on” photoelectrochemical biosensor based on cascaded quadratic amplification strategy for miRNA 141 detection. SENSORS AND ACTUATORS B-CHEMICAL, 289 (269). [10.1016/j.snb.2019.03.044]
15. Shuang Tan, Rui Han, Shilian Wu, Huan Liang, Yuting Zhao, Hui Zhao, Can-Peng Li. (2019) A novel fluorescent sensing platform for insulin detection based on competitive recognition of cationic pillar[6]arene. TALANTA, 197 (130). [PMID:30771914] [10.1016/j.talanta.2019.01.004]
16. Shaohua Ma, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Qingpeng Shen, Chunbo Liu, Zhihua Liu. (2018) Adenine/Au complex-dependent versatile electrochemical platform for ultrasensitive DNA-related enzyme activity assay. SENSORS AND ACTUATORS B-CHEMICAL, 273 (760). [10.1016/j.snb.2018.06.115]
17. Mao Mi, Tian Tian, He Yu, Ge Yili, Zhou Jiangang, Song Gongwu. (2018) Inner filter effect based fluorometric determination of the activity of alkaline phosphatase by using carbon dots codoped with boron and nitrogen. MICROCHIMICA ACTA, 185 (1): (1-6). [PMID:29594532] [10.1007/s00604-017-2541-4]
18. Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Chunnuan Du, Chunyang Zhai. (2017) In situ grown DNA nanotail-templated silver nanoclusters enabling label-free electrochemical sensing of terminal deoxynucleotidyl transferase activity. BIOSENSORS & BIOELECTRONICS, 98 (91). [PMID:28662471] [10.1016/j.bios.2017.06.017]
19. Xiangying Sun, Bin Liu, Chuanxiao Yang, Congcong Li. (2014) An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 131 (288). [PMID:24835931] [10.1016/j.saa.2014.04.093]
20. Ting Cheng,Xiang Li,Peng Huang,Han Wang,Meixia Wang,Wenming Yang. (2019-01-12) Colorimetric and electrochemical (dual) thrombin assay based on the use of a platinum nanoparticle modified metal-organic framework (type Fe-MIL-88) acting as a peroxidase mimic.. Mikrochimica acta, 186 ((2)): (94-94). [PMID:30631938]

参考文献

1. Zhechang Chen, Caijie Yuan, Yaxin Ye, Bitao Lu, Enling Hu, Fei Lu, Kun Yu, Ruiqi Xie, Guangqian Lan. (2024) Dual-targeting fucoidan-based microvesicle for arterial thrombolysis and re-occlusion inhibition. CARBOHYDRATE POLYMERS, 328 (121703). [PMID:38220339] [10.1016/j.carbpol.2023.121703]
2. Hong Liu, Xuliang Yang, Bo Huang, Hongxiang Liu. (2023) A universal approach for synthesis of copper nanoclusters templated by G-rich oligonucleotide sequences and their applications in sensing. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 297 (122740). [PMID:37080047] [10.1016/j.saa.2023.122740]
3. Xinxin Ren, Qingqing Zhang, Lingxia Qin, Kaiyue Hu, Zhiyong Guo, Sui Wang, Yufang Hu. (2023) Checkerboard arranged G4 nanostructure-supported electrochemical platform and its application to unique bio-enzymes examination. BIOELECTROCHEMISTRY, 149 (108282). [PMID:36242949] [10.1016/j.bioelechem.2022.108282]
4. Xinxin Ren, Kaiyue Hu, Lingxia Qin, Di Wu, Zhiyong Guo, Sui Wang, Yufang Hu. (2022) Development of ZnO nanoflowers-assisted DNAzyme-based electrochemical platform for invertase and glucose oxidase-dominated biosensing. ANALYTICA CHIMICA ACTA, 1232 (340438). [PMID:36257731] [10.1016/j.aca.2022.340438]
5. Songzhi Xie, Chuanfei Mo, Wenxiong Cao, Shuang Xie, Shang Li, Zhanlin Zhang, Xiaohong Li. (2022) Bacteria-propelled microtubular motors for efficient penetration and targeting delivery of thrombolytic agents. Acta Biomaterialia, 142 (49). [PMID:35158079] [10.1016/j.actbio.2022.02.008]
6. Fuad Abduro Bushira, Shimeles Addisu Kitte, Yong Wang, Haijuan Li, Ping Wang, Yongdong Jin. (2021) Plasmon-Boosted Cu-Doped TiO2 Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase. ANALYTICAL CHEMISTRY, 93 (45): (15183–15191). [PMID:34743510] [10.1021/acs.analchem.1c03842]
7. Qing Zhu, Ping Ye, Fang Guo, Yimen Zhu, Wenbin Nan, Zhaohua Chang. (2021) A heparin-functionalized covered stent prepared by plasma technology. JOURNAL OF BIOMATERIALS APPLICATIONS, [PMID:34672223] [10.1177/08853282211051871]
8. Liu Shanshan, Zheng Sitian, Chu Jing. (2021) Cationic Polythiophene-based Colorimetric Assay for Probing the Activity of Protein Kinase A. ANALYTICAL SCIENCES, 37 (7): (1039-1043). [PMID:33250451] [10.2116/analsci.20N034]
9. Zheng Di-Wei, Hong Sheng, Zhang Qiu-Ling, Dong Xue, Pan Pei, Song Wen-Fang, Song Wen, Cheng Si-Xue, Zhang Xian-Zheng. (2020) Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies. Nature Communications, 11 (1): (1-15). [PMID:32999289] [10.1038/s41467-020-18493-7]
10. Zhang Hong, Liu Pengfei, Wang Huifang, Ji Xiaoming, Zhao Mingqin, Song Zhaopeng. (2021) Label-free fluorescent sensor for one-step lysozyme detection via positively charged gold nanorods. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 413 (6): (1541-1547). [PMID:32705288] [10.1007/s00216-020-02814-2]
11. Liping Lin, Yaxin Luo, Qiushuang Chen, Qingjiao Lai, Qiaoling Zheng. (2020) Redox-modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles. LUMINESCENCE, 35 (4): (542-549). [PMID:31898408] [10.1002/bio.3749]
12. Ding Wang, Yaqin Chai, Yali Yuan, Ruo Yuan. (2020) Lattice-Like DNA Tetrahedron Nanostructure as Scaffold to Locate GOx and HRP Enzymes for Highly Efficient Enzyme Cascade Reaction. ACS Applied Materials & Interfaces, 12 (2): (2871–2877). [PMID:31849211] [10.1021/acsami.9b18702]
13. Wang Jing, Li Bingzhi, Lu Qiaoyun, Li Xiaoyun, Weng Chenyuan, Yan Xiaoqiang, Hong Junli, Zhou Xuemin. (2019) A versatile fluorometric aptasensing scheme based on the use of a hybrid material composed of polypyrrole nanoparticles and DNA-silver nanoclusters: application to the determination of adenosine, thrombin, or interferon-gamma. MICROCHIMICA ACTA, 186 (6): (1-8). [PMID:31098714] [10.1007/s00604-019-3459-9]
14. Fangjing Mo, Jingling Wu, Min Chen, Hui Meng, Qian Han, Yingzi Fu. (2019) Enzyme-free “on-off-on” photoelectrochemical biosensor based on cascaded quadratic amplification strategy for miRNA 141 detection. SENSORS AND ACTUATORS B-CHEMICAL, 289 (269). [10.1016/j.snb.2019.03.044]
15. Shuang Tan, Rui Han, Shilian Wu, Huan Liang, Yuting Zhao, Hui Zhao, Can-Peng Li. (2019) A novel fluorescent sensing platform for insulin detection based on competitive recognition of cationic pillar[6]arene. TALANTA, 197 (130). [PMID:30771914] [10.1016/j.talanta.2019.01.004]
16. Shaohua Ma, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Qingpeng Shen, Chunbo Liu, Zhihua Liu. (2018) Adenine/Au complex-dependent versatile electrochemical platform for ultrasensitive DNA-related enzyme activity assay. SENSORS AND ACTUATORS B-CHEMICAL, 273 (760). [10.1016/j.snb.2018.06.115]
17. Mao Mi, Tian Tian, He Yu, Ge Yili, Zhou Jiangang, Song Gongwu. (2018) Inner filter effect based fluorometric determination of the activity of alkaline phosphatase by using carbon dots codoped with boron and nitrogen. MICROCHIMICA ACTA, 185 (1): (1-6). [PMID:29594532] [10.1007/s00604-017-2541-4]
18. Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Chunnuan Du, Chunyang Zhai. (2017) In situ grown DNA nanotail-templated silver nanoclusters enabling label-free electrochemical sensing of terminal deoxynucleotidyl transferase activity. BIOSENSORS & BIOELECTRONICS, 98 (91). [PMID:28662471] [10.1016/j.bios.2017.06.017]
19. Xiangying Sun, Bin Liu, Chuanxiao Yang, Congcong Li. (2014) An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 131 (288). [PMID:24835931] [10.1016/j.saa.2014.04.093]
20. Ting Cheng,Xiang Li,Peng Huang,Han Wang,Meixia Wang,Wenming Yang. (2019-01-12) Colorimetric and electrochemical (dual) thrombin assay based on the use of a platinum nanoparticle modified metal-organic framework (type Fe-MIL-88) acting as a peroxidase mimic.. Mikrochimica acta, 186 ((2)): (94-94). [PMID:30631938]

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货号 (SKU)	包装规格	是否现货	价格	数量
B741818-20000US	20000US Unit	期货
B741818-100000US	100000 US Unit	期货

Bovine Thrombin

库存信息

库存信息

基本描述

产品属性

作用机制

产品规格参数

安全和危险性（GHS）

质量标准

质检证书（CoA,COO,BSE/TSE 和分析图谱)

此产品的引用文献

参考文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器