Western Blot封闭液Ⅰ(BSA)

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
B670017-100ml
100ml 现货 Stock Image
B670017-500ml
500ml 现货 Stock Image

基本描述

别名 WB封闭液(含BSA) | Western blot封闭液(含BSA) | BSA封闭液
英文别名 Blocking Western
稳定性与储存 Store at 2-8°C long term (12 months). Upon delivery aliquot.
英文名称 BSA Blocking Buffer
储存温度 2-8°C储存
运输条件 冰袋运输
产品介绍
产品介绍:
Western Blot 实验中,为防止印迹膜与检测系统中的蛋白成分(如抗体IgG等)发生非特异性吸附,导致实验结果不清晰,通常在进行一抗孵育前需预先对印迹膜上的非特异结合位点进行封闭。本产品以BSA为基础,配合其他有效成份,可快速高效地封闭膜上多余的结合部位,减少非特异结合情况的产生,降低背景,适用于各种转印膜的封闭,操作方便快捷。另外,本产品特别添加了蛋白稳定剂,可保证每次实验封闭效果稳定可靠。
注意事项:
1.每次使用时,必须保证封闭液将印迹膜完全浸没,以免影响封闭效果。
2.本产品含有防腐剂,操作时请佩戴手套进行防护。
3.本产品仅用于科研,不能用于人体实验或人体治疗。
使用说明:
1.本产品无需稀释,开盖即用。
2.目的蛋白转移完毕后,将印迹膜放入容器中,加入适量BSA Blocking Buffer(以完全覆盖印迹膜为宜)。
3.室温孵育30分钟至2小时,必要时可2-8℃孵育过夜。
4.封闭结束后弃去BSA Blocking Buffer,进行Western Blot后续操作。


Product Introduction:
In Western Blot experiments, in order to prevent non-specific adsorption between the imprinting membrane and protein components in the detection system (such as antibodies IgG, etc.), which may lead to unclear experimental results, it is usually necessary to block the non-specific binding sites on the imprinting membrane before incubating with primary antibodies. This product is based on BSA and combined with other active ingredients to quickly and efficiently seal excess binding sites on the membrane, reducing the occurrence of non-specific binding and lowering the background. It is suitable for sealing various transfer films and is easy and fast to operate. In addition, this product has specially added protein stabilizers to ensure stable and reliable sealing effects in each experiment.
Precautions:
1. During each use, it is necessary to ensure that the sealing solution completely submerges the imprinting film to avoid affecting the sealing effect.
2. This product contains preservatives, please wear gloves for protection during operation.
3. This product is only used for scientific research and cannot be used for human experiments or treatments.
Instructions for Use:
1. This product does not require dilution and can be used immediately after opening the lid.
2. After the transfer of the target protein is completed, place the imprinting membrane into a container and add an appropriate amount of BSA Blocking Buffer (preferably to completely cover the imprinting membrane).
3. Incubate at room temperature for 30 minutes to 2 hours, and if necessary, incubate overnight at 2-8 ℃.
4. After the closure is completed, discard the BSA Blocking Buffer and perform subsequent Western Blot operations.


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找到3个结果

批号(Lot Number) 证书类型 日期 货号
ZJ25F0318836 分析证书 25-03-05 B670017
ZJ25F0318835 分析证书 25-03-05 B670017
H2412073 分析证书 24-11-15 B670017

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