BCIP/NBT Stock Solution, 50X

基于碱性磷酸酶（AP）的检测系统的底物，包括蛋白质印迹

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货号 (SKU)	包装规格	是否现货	价格	数量
B385716-8ml	8ml	期货

基本描述

别名	BCIP/NBT 储备溶液，50X
规格或纯度	50 X
英文名称	BCIP/NBT Stock Solution, 50X
储存温度	2-8°C储存
运输条件	冰袋运输
产品介绍	5-溴，4-氯，3-吲哚磷酸酯（BCIP）/硝基蓝四氮唑（NBT）底物，用于定位碱性磷酸酶（AP）标记的探针。BCIP充当AP的底物，并在去磷酸化后进一步反应，得到深蓝色靛蓝染料作为氧化产物。NBT在本文中用作氧化剂，还提供深蓝色染料，增强了颜色并使检测更灵敏。 5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on Western, Northern, Southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive.\|Recommended for use with Crystal Mounting Medium (sc-24943). Preparation of staining solution:•Add 200 µl of BCIP/NBT stock solution to the following room temperature buffer: 10 ml 0.1 M Tris Hydrochloride, pH 9.5, 0.1 M Sodium Chloride, and 0.05 M Magnesium Chloride. (do not include Magnesium Chloride in the detec BCIP/NBT Stock Solution, 50Xis a substrate for alkaline phosphatase (AP) based detection systems including western blotting