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  1. Recombinant Mouse TNF RII/TNFRSF1B Protein
      种属: 小鼠(Mouse)    Accession #: P25119    
      表达系统: HEK293    蛋白标签: C-hFc & His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50 for this effect is typically 0.95 ng/mL in the presence of 0.5 ng/mL of Recombinant Mouse TNF-alpha Protein (rp154759).    
  2. Recombinant Mouse CD47 Protein
      种属: 小鼠(Mouse)    Accession #: NP_034711    
      表达系统: HEK293    蛋白标签: C-His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 binds with an apparent Kd <0.6 nM. Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 for this effect is 2.0-8.0 μg/mL. Optimal dilutions should be determined by each laboratory for each application.    
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