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  1. Recombinant Mouse CD47 Protein
      种属: 小鼠(Mouse)    Accession #: NP_034711    
      表达系统: HEK293    蛋白标签: C-His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 binds with an apparent Kd <0.6 nM. Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 for this effect is 2.0-8.0 μg/mL. Optimal dilutions should be determined by each laboratory for each application.    
  2. Recombinant Mouse Mesothelin Protein
      种属: 小鼠(Mouse)    Accession #: Q61468    
      表达系统: HEK293    蛋白标签: C-His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin C-Terminal (aa 298-600) His-tag is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.    
  3. Recombinant Mouse Ret Protein
      种属: 小鼠(Mouse)    Accession #: P35546    
      表达系统: HEK293    蛋白标签: C-His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 µL/well) was tested for binding with the GDNF-GFR alpha -1 complex using 4 ng/mL rhGDNF and 1 µg/mL rrGFR alpha -1/Fc Chimera. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2-5 µg/mL.    
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