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  1. Recombinant Human Frizzled-1 Protein
      种属: 人(Human)    Accession #: Q9UP38    
      表达系统: HEK293    蛋白标签: C-hFc & His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-1/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL. Optimal concentrations should be determined by each laboratory for each application.    
  2. Recombinant Human CD40/TNFRSF5 Protein
      种属: 人(Human)    Accession #: P25942    
      表达系统: HEK293    蛋白标签: C-His    内毒素水平: <1.0 EU/μg    
      生物活性: Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Recombinant Human CD40 Ligand Protein (rp169583) with the EC50 of 20.84 ng/mL.     Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind CD40 Mouse mAb (Ab094764) with the EC50 of 15.35 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Ravagalimab (anti-CD40) (Ab182860) with the EC50 of 11.32 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Mitazalimab (anti-CD40) (Ab182809) with the EC50 of 4.37 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Giloralimab (anti-CD40) (Ab182875) with the EC50 of 25.5 ng/mL.    
  3. Recombinant Mouse CD47 Protein
      种属: 小鼠(Mouse)    Accession #: NP_034711    
      表达系统: HEK293    蛋白标签: C-His    内毒素水平: <0.1 EU/μg    
      生物活性: Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 binds with an apparent Kd <0.6 nM. Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 for this effect is 2.0-8.0 μg/mL. Optimal dilutions should be determined by each laboratory for each application.    
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