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ZJ25R0402432计算溶液所需的质量、体积或浓度。
Goat Anti-Human IgG (LumiDye™ Blue 525) (Ab220540) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 4% PFA for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (LumiDye™ Blue 525) (Ab220540) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.