| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab219252-10μl |
10μl |
现货  |
| |
| Ab219252-50μl |
50μL |
现货 |
| |
| Ab219252-100μl |
100μL |
现货 |
| |
| Ab219252-1ml |
1ml |
期货 |
|
| 产品名称 | GAPDH Mouse mAb (Biotin) |
|---|---|
| 别名 | 重组Anti-GAPDH抗体 | 小鼠单克隆抗体 to GAPDH |
| 英文别名 | 38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP |
| 规格或纯度 | ExactAb™, 已验证, 0.5 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | GAPDH |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant full length human GAPDH |
| 阳性对照 | WB: HEK293, HeLa, Jurkat and K562 whole cell lysates Flow Cytometry: HEK293 cells ICC/IF: HeLa cells |
| 偶联 | Biotin |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 0.5 mg/mL |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20°C. Protect from light. Avoid freeze/thaw cycles. |
| 分子类型 | 抗体 |
GAPDH Mouse mAb (Biotin) (Ab219252) - Western Blot
All lanes: GAPDH Mouse mAb (Biotin) (Ab219252) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 127.5 s
GAPDH Mouse mAb (Biotin) (Ab219252) - Flow Cytometry
Flow cytometry analysis of GAPDH expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with GAPDH Mouse mAb (Biotin) (Ab219252) (Red). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
GAPDH Mouse mAb (Biotin) (Ab219252) – IF/ICC
IF analysis of GAPDH (green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Biotin) (Ab219252) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/100-1/1000
|
| Flow Cytometry | 1/1000 - 1/10000 |
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| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25R0402345 |
分析证书 | 25-04-13 | Ab219252 |
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