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Lamin A/C Mouse mAb (Biotin)

Lamin A/C Mouse mAb (Biotin)

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry
IF/ICC
WB

功能和特点

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat)
亚型: Mouse IgG2b
克隆号: AB07/4G6
偶联: Biotin

有货

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货号 (SKU)	包装规格	是否现货
Ab219209-10μl	10μl	现货
Ab219209-50μl	50μL	现货
Ab219209-100μl	100μL	现货
Ab219209-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: LMNA

Accession#:P02545 (5) Gene ID:4000 (5) LMN1 (1) LMNA (5) 生物素 (221)

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基本信息

产品名称	Lamin A/C Mouse mAb (Biotin)
别名	核纤层蛋白 \| 核纤层蛋白A \| 核纤层蛋白C \| Anti- Lamin A/C抗体 \| LMNA小鼠单克隆抗体 \| Lamin A/C小鼠单克隆抗体 \| Lamin A/C抗体 \| LMNA抗体
英文别名	70 kDa lamin antibody \| Cardiomyopathy dilated 1A (autosomal dominant) antibody \| CDCD1 antibody \| CDDC antibody \| CMD1A antibody \| CMT2B1 antibody \| EMD2 antibody \| FPL antibody \| FPLD antibody \| FPLD2 antibody \| HGPS antibody \| IDC antibody \| Lamin A an
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
宿主种属	小鼠(Mouse)
特异性	Lamin A
种属反应性	人(Human),大鼠(Rat)
免疫原	Recombinant protein corresponding to Human Lamin A
阳性对照	WB: WB: HeLa, C6 whole cell lysates Flow Cytometry: HeLa cells ICC/IF: HeLa cells
偶联	Biotin

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
分子类型	抗体

图片

ICC/IF: Lamin A/C Mouse mAb (Biotin) (Ab219209)

Lamin A/C Mouse mAb (Biotin) (Ab219209) - ICC/IF
IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (Biotin) (Ab219209) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Lamin A/C Mouse mAb (Biotin) (Ab219209)

Lamin A/C Mouse mAb (Biotin) (Ab219209) - Flow Cytometry
Flow cytometry analysis of Lamin A/C expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Lamin A/C Mouse mAb (Biotin) (Ab219209) (Red). Then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Western Blot: Lamin A/C Mouse mAb (Biotin) (Ab219209)

Lamin A/C Mouse mAb (Biotin) (Ab219209) - Western Blot
All lanes: Lamin A/C Mouse mAb (Biotin) (Ab219209) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 65, 72 kDa
Observed band size: 63, 70 kDa
Exposure time: 120.0 s