计算溶液所需的质量、体积或浓度。
Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Recommend adding 10mL of Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) solution for each mini gel. Note that the membrane needs to be totally immersed in solution. We used 4mL of ready-to-use reagent to incubate the PVDF membrane (8cm x 4cm) in this experiment.
SA-HRP: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 40 kDa
Exposure time: 100.0 s
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