CD86 Rat mAb (AF488)

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

功能和特点

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2a
克隆号: GL1
偶联: AF488

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab215320-10μg	10μg	现货
Ab215320-25μg	25μg	现货
Ab215320-100μg	100μg	现货

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点击查看靶蛋白信息: CD86

Accession#:P42082 (13) AF488 (56) B7 (12) B7-2 (18) B7.2 (16) B70 (16) Cd28l2 (12) CD86 (16) CLS1 (12) ETC-1 (12) Gene ID:12524 (13) Ly-58 (12) Ly58 (12) MB7 (12) MB7-2 (12) TS/A-2 (12)

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基本信息

产品名称	CD86 Rat mAb (AF488)
别名	CD86单克隆抗体 \| Anti-CD86抗体 \| CD86抗体 \| CD86单抗
英文别名	Activation B7-2 antigen \| B70 \| B7-2 antigen \| B72 \| B7-2 \| B-lymphocyte activation antigen B7-2 \| BU63 \| CD28 antigen ligand 2 \| CD28LG2B7-2 antigen) \| CD86 antigen \| CD86 molecule \| CD86 \| CTLA-4 counter-receptor B7.2 \| FUN-1 \| LAB72 \| MGC34413 \| T-lymp
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
宿主种属	大鼠(Rat)
特异性	CD86
种属反应性	小鼠(Mouse)
免疫原	LPS-activated CBA/Ca mouse splenic B cells
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: CD86 Rat mAb (AF488) (Ab215320)

CD86 Rat mAb (AF488) (Ab215320) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD86 Rat mAb (AF488) (Ab215320) (red) compared to an isotype control (blue). Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (AF488) (Ab215320)

CD86 Rat mAb (AF488) (Ab215320) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD86 Rat mAb (AF488) (Ab215320) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

关联靶点（人）

CD86 Tclin T淋巴细胞活化抗原CD86(T-lymphocyte activation antigen CD86) (0 活性数据)

点击查看靶蛋白信息： CD86

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

安全和危险性（GHS）

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.