基本信息

产品名称	CD46 Mouse mAb
别名	膜辅蛋白 \| Anti-CD46抗体 \| CD46小鼠单克隆抗体 \| 小鼠抗人CD46抗体 \| CD46抗体 \| Anti-MCP抗体 \| MCP小鼠单克隆抗体 \| 小鼠抗人MCP抗体 \| MCP抗体 \| Anti-MIC10抗体 \| MIC10小鼠单克隆抗体 \| 小鼠抗人MIC10抗体 \| MIC10抗体
英文别名	AHUS2 antibody \| Antigen defined by monoclonal antibody TRA 2 10 antibody \| Antigen identified by monoclonal antibody TRA 2 10 antibody \| CD46 antibody \| CD46 antigen antibody \| CD46 antigen complement regulatory protein antibody \| CD46 molecule antibody
规格或纯度	ExactAb™, 已验证, 无动物源, 无载体, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells. (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3. (Microbial infection) Acts as a receptor for cultured Measles virus. (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6. (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes.
宿主种属	小鼠(Mouse)
特异性	Human CD46
种属反应性	人(Human)
免疫原	Recombinant protein corresponding to Human CD46
阳性对照	WB: HeLa, K562, A431, A549 and MCF-7 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

图片

Flow Cytometry: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with CD46 Mouse mAb (Ab210969) (Red) at 0.125 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).

Flow Cytometry: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with CD46 Mouse mAb (Ab210969) (Red) at 0.0625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).

ICC/IF: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - ICC/IF
IF analysis of CD46 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with CD46 Mouse mAb (Ab210969) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.