| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab210603-10μg |
10μg |
现货  |
| |
| Ab210603-50μg |
50μg |
现货 |
| |
| Ab210603-100μg |
100μg |
期货 |
| |
| Ab210603-1mg |
1mg |
期货 |
|
| 产品名称 | Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody |
|---|---|
| 别名 | Flag标签抗体 | DYKDDDDK表位标签 | DYKDDDDK抗体 | Flag标签抗体 | DYKDDDDK表位标签 | DYKDDDDK抗体 |
| 英文别名 | DDDDK epitope tag antibody | DDDK antibody | ddk antibody | DYKDDDDK (also known as the FLAG® tag sequence) antibody | DYKDDDDK antibody | DYKDDDDK epitope tag antibody | DYKDDDDK tag antibody | ECS epitope tag antibody | ECS tag antibody | Enterokinase C |
| 规格或纯度 | 无动物源, 无载体, 重组, 高性能, 无叠氮钠, 已验证, ExactAb™, ≥95%(SDS-PAGE), 1.0 mg/mL |
| 生化机理 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | DYKDDDDK epitope tag |
| 种属反应性 | 不依赖种属(Species independent) |
| 免疫原 | Synthetic DYKDDDDK peptide |
| 阳性对照 | WB: DYKDDDDK tag transfected HEK293, whole cell lysate Flow Cytometry: HEK293 cells transfected with Flag tag IF/ICC: HEK293 cells transfected with Flag and mCherry tags, HEK293 cells transfected with Flag and eGFP tags |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150.0 KDa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | PBS, pH 7.4 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| 分子类型 | 抗体 |
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - Western Blot
All lanes: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 115 kDa
Exposure time: 73.3 s
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - Flow Cytometry
Overlay histogram showing HEK293T cells transfected with a EGFP-DYKDDDDK tag using Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) (Right panel). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG, Fcγ fragment specific (AF647) was used as the secondary antibody. The mouse IgG (Ab170221) was used as an isotype control (Left panel).
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - ICC/IF
IF analysis of 293T cells transfected with a EGFP-DYKDDDDK tag (Green) using Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) (Red). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - ICC/IF
IF analysis of 293T cells transfected with a mCherry-DYKDDDDK tag (Red) using Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) (Green). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC | 1/200 |
| WB |
1/1000 - 1/20000
|
| Flow Cytometry | 1/200 – 1/20000 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25R0402286 |
分析证书 | 25-04-14 | Ab210603 |
| ZJ24F1215558 |
分析证书 | 24-12-18 | Ab210603 |
| ZJ24F1215557 |
分析证书 | 24-12-18 | Ab210603 |
| ZJ24F1215556 |
分析证书 | 24-12-18 | Ab210603 |
| ZJ24F1215555 |
分析证书 | 24-12-18 | Ab210603 |
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